Isolated, perfused neontal rat heart preparation for studies of calcium and functional stability

Emma Riva, David J. Hearse

Research output: Contribution to journalArticlepeer-review

Abstract

We describe an isolated, perfused preparation for neonatal rat hearts to assess the relationship between extracellular calcium and (1) cardiac function and (2) contractile stability over 2 hours of perfusion. Neonatal (3 to 5 days old) rat hearts (n = 6 per group) were perfused for 30 minutes (Langendorff) with oxygenated buffer (37 °C) containing 1.4 mmol/L calcium (control period) and 90 minutes with buffer containing 0.5, 0.8, 1.0, 1.2, 1.4, 1.8, or 2.5 mmol/L calcium. Upon changing from 1.4 mmol/L to either a higher or low calcium concentration there were no significant changes in left ventricular developed pressure, heart rate, or coronary flow. However, left ventricular developed pressure progressively deteriorated in a time-dependent and calcium-dependent manner. Thus, after 90 minutes, developed pressure fell to 18% ± 2%, 27% ± 3%, 41% ± 5%, 47% ± 8%, 55% ± 10%, 64% ± 5% and 76% ± 4% of its initial value with 0.5, 0.8, 1.0, 1.2, 1.4, 1.8, and 2.5 mmol/L calcium. In conclusion, in studies with the neonatal rat heart, extracellular calcium concentrations in the range of 1.8 to 2.5 mmol/L are recommended.

Original languageEnglish
Pages (from-to)987-992
Number of pages6
JournalAnnals of Thoracic Surgery
Volume52
Issue number4
DOIs
Publication statusPublished - 1991

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Surgery

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