Isolation and biochemical characterization of the soluble and membrane forms of folate binding protein expressed in the ovarian carcinoma cell line IGROV1

Antonella Tomassetti, Leslie R. Coney, Silvana Canevari, Silvia Miotti, Patrizia Facheris, Vincent R. Zurawski, Maria I. Colnaghi

Research output: Contribution to journalArticlepeer-review

Abstract

The human ovarian carcinoma cell line, IGROV1, produces two forms of folate binding protein (FBP), the membrane form that is anchored to the cell surface by a glycosylphosphatidylinositol tail and the soluble form that is shed into the tissue culture medium. Both forms are recognized by the monoclonal antibodies MOv18 and MOv19. Here we describe their purification and biochemical characterization. The purified soluble protein appeared as a single band with an apparent Mr of 36 kDa after SDS-PAGE, whereas the membrane form appeared as a single band with an apparent Mr of 38 kDa. The size difference between the two forms of FBP was confirmed by gel filtration of both the native and the N-glycanase-treated proteins. Both purified proteins had equal capacity to bind folic acid. The immunological cross-reactivity and the folic acid binding capability of the FBPs extracted from IGROV1 gave more evidence of the possible existence of a precursor-product relationship between them.

Original languageEnglish
Pages (from-to)143-146
Number of pages4
JournalFEBS Letters
Volume317
Issue number1-2
DOIs
Publication statusPublished - Feb 8 1993

Keywords

  • Folate binding protein
  • Glycosylphosphatidylinositol linkage
  • Ovarian carcinoma

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology
  • Cell Biology
  • Genetics
  • Structural Biology

Fingerprint

Dive into the research topics of 'Isolation and biochemical characterization of the soluble and membrane forms of folate binding protein expressed in the ovarian carcinoma cell line IGROV1'. Together they form a unique fingerprint.

Cite this