Isolation and characterization of a spontaneously immortalized multipotent mesenchymal cell line derived from mouse subcutaneous adipose tissue

Andrea Zamperone, Stefano Pietronave, Simone Merlin, Donato Colangelo, Gabriella Ranaldo, Enzo Medico, Federica Di Scipio, Giovanni Nicolao Berta, Antonia Follenzi, Maria Prat

Research output: Contribution to journalArticle

Abstract

The emerging field of tissue engineering and regenerative medicine is a multidisciplinary science that is based on the combination of a reliable source of stem cells, biomaterial scaffolds, and cytokine growth factors. Adult mesenchymal stem cells are considered important cells for applications in this field, and adipose tissue has revealed to be an excellent source of them. Indeed, adipose-derived stem cells (ASCs) can be easily isolated from the stromal vascular fraction (SVF) of adipose tissue. During the isolation and propagation of murine ASCs, we observed the appearance of a spontaneously immortalized cell clone, named m17.ASC. This clone has been propagated for more than 180 passages and stably expresses a variety of stemness markers, such as Sca-1, c-kit/CD117, CD44, CD106, islet-1, nestin, and nucleostemin. Furthermore, these cells can be induced to differentiate toward osteogenic, chondrogenic, adipogenic, and cardiogenic phenotypes. m17.ASC clone displays a normal karyotype and stable telomeres; it neither proliferates when plated in soft agar nor gives rise to tumors when injected subcutaneously in NOD/SCID-γ null mice. The analysis of gene expression highlighted transcriptional traits of SVF cells. m17.ASCs were genetically modified by lentiviral vectors carrying green fluorescent protein (GFP) as a marker transgene and efficiently engrafted in the liver, when injected in the spleen of NOD/SCID-γ null monocrotaline-treated mice. These results suggest that this non-tumorigenic spontaneously immortalized ASC line may represent a useful tool (cell model) for studying the differentiation mechanisms involved in tissue repair as well as a model for pharmacological/toxicological studies.

Original languageEnglish
Pages (from-to)2873-2884
Number of pages12
JournalStem Cells and Development
Volume22
Issue number21
DOIs
Publication statusPublished - Nov 1 2013

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Hematology

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    Zamperone, A., Pietronave, S., Merlin, S., Colangelo, D., Ranaldo, G., Medico, E., Di Scipio, F., Berta, G. N., Follenzi, A., & Prat, M. (2013). Isolation and characterization of a spontaneously immortalized multipotent mesenchymal cell line derived from mouse subcutaneous adipose tissue. Stem Cells and Development, 22(21), 2873-2884. https://doi.org/10.1089/scd.2012.0718