Isolation and characterization of a tRNA(guanine-7-)-methyltransferase from Salmonella typhimurium

Alfredo Colonna, Gennaro Ciliberto, Rita Santamaria, Filiberto Cimino, Francesco Salvatore

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The tRNA modifying enzyme, S-adenosylmethionine:tRNA(guanine-7-)-methyltransferase, has been extensively purified from Salmonella typhimurium. A rapid and efficient purification method using phosphocellulose chromatography followed by ammonium sulfate precipitation and Sephadex G-100 gel filtration is described. The enzyme appears to be a single polypeptide chain with a molecular weight of approximately 25 000-30 000 daltons. The Km for S-adenosylmethionine and for undermethylated tRNA is 53 μ M and 3.4 μM, respectively. The methylation reaction is dependent on added monovalent or divalent cations; 5 mM spermidine, 3 mM MgCl2 and 1 mM spermine are the most effective. The enzyme, though not homogeneous, is free from contaminating ribonucleases and other tRNA methyltransferases.

Original languageEnglish
Pages (from-to)97-106
Number of pages10
JournalMolecular and Cellular Biochemistry
Volume52
Issue number2
DOIs
Publication statusPublished - Sep 1983

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Salmonella
Salmonella typhimurium
S-Adenosylmethionine
Transfer RNA
tRNA Methyltransferases
Enzymes
Monovalent Cations
Magnesium Chloride
Methylation
Spermidine
Spermine
Divalent Cations
Ammonium Sulfate
Ribonucleases
Chromatography
Purification
Gel Chromatography
Molecular Weight
Gels
Molecular weight

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Clinical Biochemistry
  • Cell Biology

Cite this

Isolation and characterization of a tRNA(guanine-7-)-methyltransferase from Salmonella typhimurium. / Colonna, Alfredo; Ciliberto, Gennaro; Santamaria, Rita; Cimino, Filiberto; Salvatore, Francesco.

In: Molecular and Cellular Biochemistry, Vol. 52, No. 2, 09.1983, p. 97-106.

Research output: Contribution to journalArticle

Colonna, Alfredo ; Ciliberto, Gennaro ; Santamaria, Rita ; Cimino, Filiberto ; Salvatore, Francesco. / Isolation and characterization of a tRNA(guanine-7-)-methyltransferase from Salmonella typhimurium. In: Molecular and Cellular Biochemistry. 1983 ; Vol. 52, No. 2. pp. 97-106.
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