Isolation and characterization of cardiac mesenchymal stromal cells from endomyocardial bioptic samples of arrhythmogenic cardiomyopathy patients

Research output: Contribution to journalArticle

Abstract

A normal adult heart is composed of several different cell types, among which cardiac mesenchymal stromal cells represent an abundant population. The isolation of these cells offers the possibility of studying their involvement in cardiac diseases, and, in addition, provides a useful primary cell model to investigate biological mechanisms. Here, the method for the isolation of C-MSC from arrhythmogenic cardiomyopathy patients’ bioptic samples is described. The endomyocardial biopsy sampling is guided in the right ventricular areas adjacent to the scar visualized by electro-anatomical mapping. The digestion of the biopsies in collagenase and their plating on a plastic dish in culture medium to allow C-MSC growth is described. The isolated cells can be expanded in culture for several passages. To confirm their mesenchymal phenotype, the description of immuno-phenotypical characterization is provided. C-MSC are able to differentiate into several cell types like adipocytes, chondrocytes, and osteoblasts: in the context of ACM, characterized by adipocyte deposits in patients’ hearts, the protocols for the adipogenic differentiation of C-MSC and the characterization of lipid droplet accumulation are described.

Original languageEnglish
Article numbere57263
JournalJournal of Visualized Experiments
Volume2018
Issue number132
DOIs
Publication statusPublished - Jan 1 2018

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Biopsy
Mesenchymal Stromal Cells
Cardiomyopathies
Osteoblasts
Collagenases
Plating
Adipocytes
Lipids
Culture Media
Deposits
Sampling
Plastics
Cell Separation
Chondrocytes
Cicatrix
Digestion
Heart Diseases
Phenotype
Growth
Population

Keywords

  • Adipogenesis
  • Arrhythmogenic Cardiomyopathy
  • ARVC
  • Cardiac mesenchymal stromal cells
  • Characterization
  • Developmental biology
  • Differentiation
  • Endomyocardial biopsy
  • Isolation
  • Issue 132

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

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abstract = "A normal adult heart is composed of several different cell types, among which cardiac mesenchymal stromal cells represent an abundant population. The isolation of these cells offers the possibility of studying their involvement in cardiac diseases, and, in addition, provides a useful primary cell model to investigate biological mechanisms. Here, the method for the isolation of C-MSC from arrhythmogenic cardiomyopathy patients’ bioptic samples is described. The endomyocardial biopsy sampling is guided in the right ventricular areas adjacent to the scar visualized by electro-anatomical mapping. The digestion of the biopsies in collagenase and their plating on a plastic dish in culture medium to allow C-MSC growth is described. The isolated cells can be expanded in culture for several passages. To confirm their mesenchymal phenotype, the description of immuno-phenotypical characterization is provided. C-MSC are able to differentiate into several cell types like adipocytes, chondrocytes, and osteoblasts: in the context of ACM, characterized by adipocyte deposits in patients’ hearts, the protocols for the adipogenic differentiation of C-MSC and the characterization of lipid droplet accumulation are described.",
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AU - Pilato, Chiara Assunta

AU - Stadiotti, Ilaria

AU - Maione, Angela Serena

AU - Saverio, Valentina

AU - Catto, Valentina

AU - Tundo, Fabrizio

AU - Russo, Antonio Dello

AU - Tondo, Claudio

AU - Pompilio, Giulio

AU - Casella, Michela

AU - Sommariva, Elena

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