Isolation and characterization of exosomes derived from fertile sheep hydatid cysts

Mar Siles-Lucas, Carlos Sánchez-Ovejero, María González-Sánchez, Esperanza González, Juan Manuel Falcón-Pérez, Belgees Boufana, Federica Fratini, Adriano Casulli, Raúl Manzano-Román

Research output: Contribution to journalArticle

Abstract

Cystic echinococcosis (CE) is a chronic and complex zoonotic disease. Information on the mechanisms involved in parasite establishment, growth and persistence remain limited. These may be modulated by a crosstalk between extracellular vesicles (EVs). EVs including exosomes and microvesicles are able to carry developmental signaling proteins which coordinate growth and establishment of several parasites. Here, an exosome enriched EV fraction was isolated from hydatid fluid (HF) of fertile sheep cysts. A proteomic analysis of this fraction identified a number of parasite-derived vesicle-membrane associated proteins as well as cytosolic proteins. Additionally, the exosomal enriched fraction contained proteins of host origin. Specific proteins −antigen B2 and TSPAN14- in the exosomal fraction were further assayed by immunoblot and transmission electron microscopy. To the best of our knowledge, this is the first report on the presence of parasite exosomes in fertile hydatid cyst fluid. Further characterization of the exosome cargo will allow the discovery of new markers for the detection of CE in humans and animals, and the treatment of CE patients, and provide new insights regarding the role of these EVs in the establishment and persistence of hydatid cysts.

Original languageEnglish
Pages (from-to)22-33
Number of pages12
JournalVeterinary Parasitology
Volume236
DOIs
Publication statusPublished - Mar 15 2017

Fingerprint

exosomes
Exosomes
hydatids
Echinococcosis
Sheep
echinococcosis
Parasites
sheep
parasites
proteins
Proteins
R-SNARE Proteins
Cyst Fluid
Zoonoses
zoonoses
Growth
Transmission Electron Microscopy
Proteomics
proteomics
transmission electron microscopy

Keywords

  • Cystic echinococcosis
  • Echinococcus granulosus
  • Exosomes
  • Extracellular vesicles
  • Proteomics
  • Virulence factors

ASJC Scopus subject areas

  • Parasitology
  • veterinary(all)

Cite this

Siles-Lucas, M., Sánchez-Ovejero, C., González-Sánchez, M., González, E., Falcón-Pérez, J. M., Boufana, B., ... Manzano-Román, R. (2017). Isolation and characterization of exosomes derived from fertile sheep hydatid cysts. Veterinary Parasitology, 236, 22-33. https://doi.org/10.1016/j.vetpar.2017.01.022

Isolation and characterization of exosomes derived from fertile sheep hydatid cysts. / Siles-Lucas, Mar; Sánchez-Ovejero, Carlos; González-Sánchez, María; González, Esperanza; Falcón-Pérez, Juan Manuel; Boufana, Belgees; Fratini, Federica; Casulli, Adriano; Manzano-Román, Raúl.

In: Veterinary Parasitology, Vol. 236, 15.03.2017, p. 22-33.

Research output: Contribution to journalArticle

Siles-Lucas, M, Sánchez-Ovejero, C, González-Sánchez, M, González, E, Falcón-Pérez, JM, Boufana, B, Fratini, F, Casulli, A & Manzano-Román, R 2017, 'Isolation and characterization of exosomes derived from fertile sheep hydatid cysts', Veterinary Parasitology, vol. 236, pp. 22-33. https://doi.org/10.1016/j.vetpar.2017.01.022
Siles-Lucas M, Sánchez-Ovejero C, González-Sánchez M, González E, Falcón-Pérez JM, Boufana B et al. Isolation and characterization of exosomes derived from fertile sheep hydatid cysts. Veterinary Parasitology. 2017 Mar 15;236:22-33. https://doi.org/10.1016/j.vetpar.2017.01.022
Siles-Lucas, Mar ; Sánchez-Ovejero, Carlos ; González-Sánchez, María ; González, Esperanza ; Falcón-Pérez, Juan Manuel ; Boufana, Belgees ; Fratini, Federica ; Casulli, Adriano ; Manzano-Román, Raúl. / Isolation and characterization of exosomes derived from fertile sheep hydatid cysts. In: Veterinary Parasitology. 2017 ; Vol. 236. pp. 22-33.
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