Isolation and characterization of human breast tumor-derived endothelial cells

Cristina Grange, Benedetta Bussolati, Stefania Bruno, Valentina Fonsato, Anna Sapino, Giovanni Camussi

Research output: Contribution to journalArticle

Abstract

Increasing evidence indicates that tumor-derived endothelial cells (TEC) possess a distinct and unique phenotype in respect to normal endothelial cells and may be able to acquire resistance to drugs. However, few functional studies are available on cultured TEC. In the present study, we obtained TEC from human breast carcinomas and, to dispel the possibility of contaminating tumor cells, we established six different clones that we characterized at a functional level. Breast TEC cell lines and clones did not undergo normal cell senescence in culture and showed constant expression of markers of endothelial activation and angiogenesis. These cells showed increased apoptosis resistance to vincristine and doxorubicin and increased random cell motility, as compared to normal micro-endothelial cells. In addition, breast TEC, at variance to normal endothelial cells, were able to grow and to organize in the absence of serum in capillary-like structures on Matrigel that persisted up to one week. These functional characteristics of breast TEC may be relevant for tumor angiogenesis and may indicate an increased pro-angiogenic activity of endothelial cells within the tumor. Moreover, our data suggest that TEC might be more appropriate for screening antiangiogenic drugs than normal endothelial cells.

Original languageEnglish
Pages (from-to)381-386
Number of pages6
JournalOncology Reports
Volume15
Issue number2
Publication statusPublished - Feb 2006

    Fingerprint

Keywords

  • Angiogenesis
  • Apoptosis resistance
  • Breast carcinoma
  • Tumor-derived endothelial cells

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Grange, C., Bussolati, B., Bruno, S., Fonsato, V., Sapino, A., & Camussi, G. (2006). Isolation and characterization of human breast tumor-derived endothelial cells. Oncology Reports, 15(2), 381-386.