Isolation and characterization of human breast tumor-derived endothelial cells

Cristina Grange, Benedetta Bussolati, Stefania Bruno, Valentina Fonsato, Anna Sapino, Giovanni Camussi

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

Increasing evidence indicates that tumor-derived endothelial cells (TEC) possess a distinct and unique phenotype in respect to normal endothelial cells and may be able to acquire resistance to drugs. However, few functional studies are available on cultured TEC. In the present study, we obtained TEC from human breast carcinomas and, to dispel the possibility of contaminating tumor cells, we established six different clones that we characterized at a functional level. Breast TEC cell lines and clones did not undergo normal cell senescence in culture and showed constant expression of markers of endothelial activation and angiogenesis. These cells showed increased apoptosis resistance to vincristine and doxorubicin and increased random cell motility, as compared to normal micro-endothelial cells. In addition, breast TEC, at variance to normal endothelial cells, were able to grow and to organize in the absence of serum in capillary-like structures on Matrigel that persisted up to one week. These functional characteristics of breast TEC may be relevant for tumor angiogenesis and may indicate an increased pro-angiogenic activity of endothelial cells within the tumor. Moreover, our data suggest that TEC might be more appropriate for screening antiangiogenic drugs than normal endothelial cells.

Original languageEnglish
Pages (from-to)381-386
Number of pages6
JournalOncology Reports
Volume15
Issue number2
Publication statusPublished - Feb 2006

Fingerprint

Endothelial Cells
Breast Neoplasms
Neoplasms
Clone Cells
Preclinical Drug Evaluations
Cell Aging
Vincristine
Drug Resistance
Doxorubicin
Cell Movement
Apoptosis
Phenotype
Cell Line
Serum

Keywords

  • Angiogenesis
  • Apoptosis resistance
  • Breast carcinoma
  • Tumor-derived endothelial cells

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Grange, C., Bussolati, B., Bruno, S., Fonsato, V., Sapino, A., & Camussi, G. (2006). Isolation and characterization of human breast tumor-derived endothelial cells. Oncology Reports, 15(2), 381-386.

Isolation and characterization of human breast tumor-derived endothelial cells. / Grange, Cristina; Bussolati, Benedetta; Bruno, Stefania; Fonsato, Valentina; Sapino, Anna; Camussi, Giovanni.

In: Oncology Reports, Vol. 15, No. 2, 02.2006, p. 381-386.

Research output: Contribution to journalArticle

Grange, C, Bussolati, B, Bruno, S, Fonsato, V, Sapino, A & Camussi, G 2006, 'Isolation and characterization of human breast tumor-derived endothelial cells', Oncology Reports, vol. 15, no. 2, pp. 381-386.
Grange C, Bussolati B, Bruno S, Fonsato V, Sapino A, Camussi G. Isolation and characterization of human breast tumor-derived endothelial cells. Oncology Reports. 2006 Feb;15(2):381-386.
Grange, Cristina ; Bussolati, Benedetta ; Bruno, Stefania ; Fonsato, Valentina ; Sapino, Anna ; Camussi, Giovanni. / Isolation and characterization of human breast tumor-derived endothelial cells. In: Oncology Reports. 2006 ; Vol. 15, No. 2. pp. 381-386.
@article{10fa3a74ee3f4b9ebafa8598167118ae,
title = "Isolation and characterization of human breast tumor-derived endothelial cells",
abstract = "Increasing evidence indicates that tumor-derived endothelial cells (TEC) possess a distinct and unique phenotype in respect to normal endothelial cells and may be able to acquire resistance to drugs. However, few functional studies are available on cultured TEC. In the present study, we obtained TEC from human breast carcinomas and, to dispel the possibility of contaminating tumor cells, we established six different clones that we characterized at a functional level. Breast TEC cell lines and clones did not undergo normal cell senescence in culture and showed constant expression of markers of endothelial activation and angiogenesis. These cells showed increased apoptosis resistance to vincristine and doxorubicin and increased random cell motility, as compared to normal micro-endothelial cells. In addition, breast TEC, at variance to normal endothelial cells, were able to grow and to organize in the absence of serum in capillary-like structures on Matrigel that persisted up to one week. These functional characteristics of breast TEC may be relevant for tumor angiogenesis and may indicate an increased pro-angiogenic activity of endothelial cells within the tumor. Moreover, our data suggest that TEC might be more appropriate for screening antiangiogenic drugs than normal endothelial cells.",
keywords = "Angiogenesis, Apoptosis resistance, Breast carcinoma, Tumor-derived endothelial cells",
author = "Cristina Grange and Benedetta Bussolati and Stefania Bruno and Valentina Fonsato and Anna Sapino and Giovanni Camussi",
year = "2006",
month = "2",
language = "English",
volume = "15",
pages = "381--386",
journal = "Oncology Reports",
issn = "1021-335X",
publisher = "Spandidos Publications",
number = "2",

}

TY - JOUR

T1 - Isolation and characterization of human breast tumor-derived endothelial cells

AU - Grange, Cristina

AU - Bussolati, Benedetta

AU - Bruno, Stefania

AU - Fonsato, Valentina

AU - Sapino, Anna

AU - Camussi, Giovanni

PY - 2006/2

Y1 - 2006/2

N2 - Increasing evidence indicates that tumor-derived endothelial cells (TEC) possess a distinct and unique phenotype in respect to normal endothelial cells and may be able to acquire resistance to drugs. However, few functional studies are available on cultured TEC. In the present study, we obtained TEC from human breast carcinomas and, to dispel the possibility of contaminating tumor cells, we established six different clones that we characterized at a functional level. Breast TEC cell lines and clones did not undergo normal cell senescence in culture and showed constant expression of markers of endothelial activation and angiogenesis. These cells showed increased apoptosis resistance to vincristine and doxorubicin and increased random cell motility, as compared to normal micro-endothelial cells. In addition, breast TEC, at variance to normal endothelial cells, were able to grow and to organize in the absence of serum in capillary-like structures on Matrigel that persisted up to one week. These functional characteristics of breast TEC may be relevant for tumor angiogenesis and may indicate an increased pro-angiogenic activity of endothelial cells within the tumor. Moreover, our data suggest that TEC might be more appropriate for screening antiangiogenic drugs than normal endothelial cells.

AB - Increasing evidence indicates that tumor-derived endothelial cells (TEC) possess a distinct and unique phenotype in respect to normal endothelial cells and may be able to acquire resistance to drugs. However, few functional studies are available on cultured TEC. In the present study, we obtained TEC from human breast carcinomas and, to dispel the possibility of contaminating tumor cells, we established six different clones that we characterized at a functional level. Breast TEC cell lines and clones did not undergo normal cell senescence in culture and showed constant expression of markers of endothelial activation and angiogenesis. These cells showed increased apoptosis resistance to vincristine and doxorubicin and increased random cell motility, as compared to normal micro-endothelial cells. In addition, breast TEC, at variance to normal endothelial cells, were able to grow and to organize in the absence of serum in capillary-like structures on Matrigel that persisted up to one week. These functional characteristics of breast TEC may be relevant for tumor angiogenesis and may indicate an increased pro-angiogenic activity of endothelial cells within the tumor. Moreover, our data suggest that TEC might be more appropriate for screening antiangiogenic drugs than normal endothelial cells.

KW - Angiogenesis

KW - Apoptosis resistance

KW - Breast carcinoma

KW - Tumor-derived endothelial cells

UR - http://www.scopus.com/inward/record.url?scp=33744470405&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33744470405&partnerID=8YFLogxK

M3 - Article

C2 - 16391858

AN - SCOPUS:33744470405

VL - 15

SP - 381

EP - 386

JO - Oncology Reports

JF - Oncology Reports

SN - 1021-335X

IS - 2

ER -