TY - JOUR
T1 - Isolation and characterization of multipotent cells from human fetal dermis
AU - Chinnici, Cinzia Maria
AU - Amico, Giandomenico
AU - Monti, Marcello
AU - Motta, Stefania
AU - Casalone, Rosario
AU - Li Petri, Sergio
AU - Spada, Marco
AU - Gridelli, Bruno
AU - Conaldi, Pier Giulio
PY - 2014
Y1 - 2014
N2 - We report that cells from human fetal dermis, termed here multipotent fetal dermal cells, can be isolated with high efficiency by using a nonenzymatic, cell outgrowth method. The resulting cell population was consistent with the definition of mesenchymal stromal cells by the International Society for Cellular Therapy. As multipotent fetal dermal cells proliferate extensively, with no loss of multilineage differentiation potential up to passage 25, they may be an ideal source for cell therapy to repair damaged tissues and organs. Multipotent fetal dermal cells were not recognized as targets by T lymphocytes in vitro, thus supporting their feasibility for allogenic transplantation. Moreover, the expansion protocol did not affect the normal phenotype and karyotype of cells. When compared with adult dermal cells, fetal cells displayed several advantages, including a greater cellular yield after isolation, the ability to proliferate longer, and the retention of differentiation potential. Interestingly, multipotent fetal dermal cells expressed the pluripotency marker SSEA4 (90.56 ± 3.15% fetal vs. 10.5 ± 8.5% adult) and coexpressed mesenchymal and epithelial markers (>80% CD90+/CK18+ cells), coexpression lacking in the adult counterparts isolated under the same conditions. Multipotent fetal dermal cells were able to form capillary structures, as well as differentiate into a simple epithelium in vitro, indicating skin regeneration capabilities.
AB - We report that cells from human fetal dermis, termed here multipotent fetal dermal cells, can be isolated with high efficiency by using a nonenzymatic, cell outgrowth method. The resulting cell population was consistent with the definition of mesenchymal stromal cells by the International Society for Cellular Therapy. As multipotent fetal dermal cells proliferate extensively, with no loss of multilineage differentiation potential up to passage 25, they may be an ideal source for cell therapy to repair damaged tissues and organs. Multipotent fetal dermal cells were not recognized as targets by T lymphocytes in vitro, thus supporting their feasibility for allogenic transplantation. Moreover, the expansion protocol did not affect the normal phenotype and karyotype of cells. When compared with adult dermal cells, fetal cells displayed several advantages, including a greater cellular yield after isolation, the ability to proliferate longer, and the retention of differentiation potential. Interestingly, multipotent fetal dermal cells expressed the pluripotency marker SSEA4 (90.56 ± 3.15% fetal vs. 10.5 ± 8.5% adult) and coexpressed mesenchymal and epithelial markers (>80% CD90+/CK18+ cells), coexpression lacking in the adult counterparts isolated under the same conditions. Multipotent fetal dermal cells were able to form capillary structures, as well as differentiate into a simple epithelium in vitro, indicating skin regeneration capabilities.
KW - Differentiation potential
KW - Human fetal skin
KW - Progenitor cells
KW - Skin regeneration
UR - http://www.scopus.com/inward/record.url?scp=84907985420&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84907985420&partnerID=8YFLogxK
U2 - 10.3727/096368913X668618
DO - 10.3727/096368913X668618
M3 - Article
C2 - 23768775
AN - SCOPUS:84907985420
VL - 23
SP - 1169
EP - 1185
JO - Cell Transplantation
JF - Cell Transplantation
SN - 0963-6897
IS - 10
ER -