Abstract
Cholecystokinin (CCK) exists in multiple molecular forms with different polypeptide lengths and the absence o-presence of sulphation. We have isolated sulphated and nonsulphated forms of CCK-58 from porcine intestine and have determined their bioactivities in a guinea-pig gallbladder contraction assay. Both forms co-eluted in cation-exchange chromatography and in several rounds of reverse-phase (RP)-HPLC, but separated upon RP-HPLC using a water/acetonitrile system with heptafluorobutyric acid as counter ion. Nonsulphated CCK-58 was the form detected by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry because of desulphation in that process. The biological activity of CCK-58 and CCK-33 is equipotent, although the kinetics of the response differ. Sulphated CCK-58 was found to be 35 times more potent than nonsulphated CCK-58. In contrast, sulphated CCK- 8 is 150 times more potent than nonsulphated CCK-8, and for sulphated and nonsulphated CCK-33, the activities differ by a factor of 100. This type of correlation indicates that the N-terminal end of CCK-58 partially compensates for the decrease in activity arising from the lack of sulphated tyrosine. Given its fairly high bioactivity, nonsulphated CCK-58 may have a physiological significance.
| Original language | English |
|---|---|
| Pages (from-to) | 336-340 |
| Number of pages | 5 |
| Journal | European Journal of Biochemistry |
| Volume | 264 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Sep 1 1999 |
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Keywords
- Cholecystokinin-58
- Gallbladder contraction assay
- MALDI mass spectrometry
ASJC Scopus subject areas
- Biochemistry
Cite this
Isolation and characterization of sulphated and nonsulphated forms of cholecystokinin-58 and their action on gallbladder contraction. / Bonetto, Valentina; Jörnvall, Hans; Andersson, Mats; Renlund, Staffan; Mutt, Viktor; Sillard, Rannar.
In: European Journal of Biochemistry, Vol. 264, No. 2, 01.09.1999, p. 336-340.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Isolation and characterization of sulphated and nonsulphated forms of cholecystokinin-58 and their action on gallbladder contraction
AU - Bonetto, Valentina
AU - Jörnvall, Hans
AU - Andersson, Mats
AU - Renlund, Staffan
AU - Mutt, Viktor
AU - Sillard, Rannar
PY - 1999/9/1
Y1 - 1999/9/1
N2 - Cholecystokinin (CCK) exists in multiple molecular forms with different polypeptide lengths and the absence o-presence of sulphation. We have isolated sulphated and nonsulphated forms of CCK-58 from porcine intestine and have determined their bioactivities in a guinea-pig gallbladder contraction assay. Both forms co-eluted in cation-exchange chromatography and in several rounds of reverse-phase (RP)-HPLC, but separated upon RP-HPLC using a water/acetonitrile system with heptafluorobutyric acid as counter ion. Nonsulphated CCK-58 was the form detected by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry because of desulphation in that process. The biological activity of CCK-58 and CCK-33 is equipotent, although the kinetics of the response differ. Sulphated CCK-58 was found to be 35 times more potent than nonsulphated CCK-58. In contrast, sulphated CCK- 8 is 150 times more potent than nonsulphated CCK-8, and for sulphated and nonsulphated CCK-33, the activities differ by a factor of 100. This type of correlation indicates that the N-terminal end of CCK-58 partially compensates for the decrease in activity arising from the lack of sulphated tyrosine. Given its fairly high bioactivity, nonsulphated CCK-58 may have a physiological significance.
AB - Cholecystokinin (CCK) exists in multiple molecular forms with different polypeptide lengths and the absence o-presence of sulphation. We have isolated sulphated and nonsulphated forms of CCK-58 from porcine intestine and have determined their bioactivities in a guinea-pig gallbladder contraction assay. Both forms co-eluted in cation-exchange chromatography and in several rounds of reverse-phase (RP)-HPLC, but separated upon RP-HPLC using a water/acetonitrile system with heptafluorobutyric acid as counter ion. Nonsulphated CCK-58 was the form detected by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry because of desulphation in that process. The biological activity of CCK-58 and CCK-33 is equipotent, although the kinetics of the response differ. Sulphated CCK-58 was found to be 35 times more potent than nonsulphated CCK-58. In contrast, sulphated CCK- 8 is 150 times more potent than nonsulphated CCK-8, and for sulphated and nonsulphated CCK-33, the activities differ by a factor of 100. This type of correlation indicates that the N-terminal end of CCK-58 partially compensates for the decrease in activity arising from the lack of sulphated tyrosine. Given its fairly high bioactivity, nonsulphated CCK-58 may have a physiological significance.
KW - Cholecystokinin-58
KW - Gallbladder contraction assay
KW - MALDI mass spectrometry
UR - http://www.scopus.com/inward/record.url?scp=0033200197&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033200197&partnerID=8YFLogxK
U2 - 10.1046/j.1432-1327.1999.00599.x
DO - 10.1046/j.1432-1327.1999.00599.x
M3 - Article
C2 - 10491077
AN - SCOPUS:0033200197
VL - 264
SP - 336
EP - 340
JO - European Journal of Biochemistry
JF - European Journal of Biochemistry
SN - 0014-2956
IS - 2
ER -