Isolation and characterization of variant cDNAs encoding mouse tyrosinase

Mineko Terao, Linda Tabe, Enrico Garattini, Daniela Sartori, Michela Studer, Beatrice Mintz

Research output: Contribution to journalArticlepeer-review

Abstract

Two different cDNA clones encoding mouse tyrosinase (monophenol oxygenase, E.C. 1.14.18.1) were isolated from B16 melanoma cells, and their primary structure was determined. One of the cDNAs consists of 3309 nucleotides with an open reading frame coding for a peptide of 533 amino acids. The other cDNA is ∼1600 nucleotides long, with a shorter 3′-untranslated region and a deduced in-frame deletion of 77 amino acid residues with respect to the former clone. Neither of these clones is structurally identical to other described mouse tyrosinase cDNAs (1-3). RNA blotting analysis demonstrates that multiple tyrosinase mRNA species are not only present in B16 melanoma, but also in normal skin melanocytes.

Original languageEnglish
Pages (from-to)848-853
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume159
Issue number2
DOIs
Publication statusPublished - Mar 15 1989

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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