Isolation and chromosomal localization of GPR31, a human gene encoding a putative G protein-coupled receptor

Alessandra Zingoni, Mariano Rocchi, Clelia Tiziana Storlazzi, Giovanni Bernardini, Angela Santoni, Monica Napolitano

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The screening of a human genomic library with a chemokine receptor-like probe allowed us to obtain a putative member of the G protein-coupled receptor gene (GPCR) family, designated GPR31. Its deduced amino acid sequence encodes a polypeptide of 319 amino acids that shares 25-33% homology with members of the chemokine, purino, and somatostatin receptor gene families. Amino acid sequence comparison reveals that the best match in the protein databases is with the human orphan GPCR called HM74 (33% identity). Southern genomic analysis of the GPR31 gene shows a hybridization pattern consistent with that of a single-copy gene. Using fluorescence in situ hybridization, we have determined the chromosomal and regional localization of the GPR31 gene at 6q27. The GPR31 mRNA is expressed at low levels by several human cell lines of different cellular origins. The phylogenetic analysis suggests that the GPR31 receptor may represent a member of a new GPCR sub-family.

Original languageEnglish
Pages (from-to)519-523
Number of pages5
JournalGenomics
Volume42
Issue number3
DOIs
Publication statusPublished - Jun 15 1997

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G-Protein-Coupled Receptors
Genes
Chemokine Receptors
Amino Acid Sequence
Somatostatin Receptors
Protein Databases
Orphaned Children
Genomic Library
Fluorescence In Situ Hybridization
Amino Acids
Cell Line
Messenger RNA
Peptides

ASJC Scopus subject areas

  • Genetics

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Isolation and chromosomal localization of GPR31, a human gene encoding a putative G protein-coupled receptor. / Zingoni, Alessandra; Rocchi, Mariano; Storlazzi, Clelia Tiziana; Bernardini, Giovanni; Santoni, Angela; Napolitano, Monica.

In: Genomics, Vol. 42, No. 3, 15.06.1997, p. 519-523.

Research output: Contribution to journalArticle

Zingoni, Alessandra ; Rocchi, Mariano ; Storlazzi, Clelia Tiziana ; Bernardini, Giovanni ; Santoni, Angela ; Napolitano, Monica. / Isolation and chromosomal localization of GPR31, a human gene encoding a putative G protein-coupled receptor. In: Genomics. 1997 ; Vol. 42, No. 3. pp. 519-523.
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