Isolation and chromosomal localization of GPR31, a human gene encoding a putative G protein-coupled receptor

Alessandra Zingoni, Mariano Rocchi, Clelia Tiziana Storlazzi, Giovanni Bernardini, Angela Santoni, Monica Napolitano

Research output: Contribution to journalArticle

Abstract

The screening of a human genomic library with a chemokine receptor-like probe allowed us to obtain a putative member of the G protein-coupled receptor gene (GPCR) family, designated GPR31. Its deduced amino acid sequence encodes a polypeptide of 319 amino acids that shares 25-33% homology with members of the chemokine, purino, and somatostatin receptor gene families. Amino acid sequence comparison reveals that the best match in the protein databases is with the human orphan GPCR called HM74 (33% identity). Southern genomic analysis of the GPR31 gene shows a hybridization pattern consistent with that of a single-copy gene. Using fluorescence in situ hybridization, we have determined the chromosomal and regional localization of the GPR31 gene at 6q27. The GPR31 mRNA is expressed at low levels by several human cell lines of different cellular origins. The phylogenetic analysis suggests that the GPR31 receptor may represent a member of a new GPCR sub-family.

Original languageEnglish
Pages (from-to)519-523
Number of pages5
JournalGenomics
Volume42
Issue number3
DOIs
Publication statusPublished - Jun 15 1997

ASJC Scopus subject areas

  • Genetics

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