Isolation and comparative mapping of a human chromosome 20-specific α-satellite DNA clone

A. Baldim, N. Archidiacono, R. Carbone, A. Bolino, V. Shridhar, O. J. Miller, D. A. Miller, D. C. Ward, M. Rocchi

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

We have isolated and characterized a human genomic DNA clone (PZ20, locus D20Z2) that identifies, under high-stringency hybridization conditions, an alphoid DNA subset specific lor chromosome 20. The specificity was determined using fluorescence in situ hybridization. Sequence analysis confirmed our previously reported data on the great similarity between the chromosome 20 and chromosome 2 alphoid subsets. Comparative mapping of pZ20 on chimpanzee and gorilla chromosomes, also performed under high-stringencv conditions. indicates that the alphoid subset has ancestral sequences on chimpanzee chromosome II and gorilla chromosome 14. I lowever. no hybridization was observed to chromosomes 21 in the great apes, the homolog of human chromosome 20.

Original languageEnglish
Pages (from-to)11-16
Number of pages6
JournalCytogenetic and Genome Research
Volume59
Issue number1
DOIs
Publication statusPublished - 1992

Fingerprint

Chromosomes, Human, Pair 20
Satellite DNA
Human Chromosomes
Gorilla gorilla
Pan troglodytes
Clone Cells
Chromosomes
Chromosomes, Human, Pair 14
Chromosomes, Human, Pair 21
Chromosomes, Human, Pair 2
DNA
Hominidae
Fluorescence In Situ Hybridization
Sequence Analysis

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Genetics(clinical)
  • Cell Biology

Cite this

Baldim, A., Archidiacono, N., Carbone, R., Bolino, A., Shridhar, V., Miller, O. J., ... Rocchi, M. (1992). Isolation and comparative mapping of a human chromosome 20-specific α-satellite DNA clone. Cytogenetic and Genome Research, 59(1), 11-16. https://doi.org/10.1159/000133188

Isolation and comparative mapping of a human chromosome 20-specific α-satellite DNA clone. / Baldim, A.; Archidiacono, N.; Carbone, R.; Bolino, A.; Shridhar, V.; Miller, O. J.; Miller, D. A.; Ward, D. C.; Rocchi, M.

In: Cytogenetic and Genome Research, Vol. 59, No. 1, 1992, p. 11-16.

Research output: Contribution to journalArticle

Baldim, A, Archidiacono, N, Carbone, R, Bolino, A, Shridhar, V, Miller, OJ, Miller, DA, Ward, DC & Rocchi, M 1992, 'Isolation and comparative mapping of a human chromosome 20-specific α-satellite DNA clone', Cytogenetic and Genome Research, vol. 59, no. 1, pp. 11-16. https://doi.org/10.1159/000133188
Baldim, A. ; Archidiacono, N. ; Carbone, R. ; Bolino, A. ; Shridhar, V. ; Miller, O. J. ; Miller, D. A. ; Ward, D. C. ; Rocchi, M. / Isolation and comparative mapping of a human chromosome 20-specific α-satellite DNA clone. In: Cytogenetic and Genome Research. 1992 ; Vol. 59, No. 1. pp. 11-16.
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