Isolation and expansion of brain microvascular endothelial cells

Stefania Elena Navone, Giovanni Marfia, Giulio Alessandri

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Here, we describe immunohistochemical methods to stain murine adipose tissue to study the structure of adipose vasculature and angiogenesis. This chapter describes an n vivo model to study adipose angiogenesis in mice. As opposed to in vitro angiogenesis assays, the described model recapitulates vascular alterations as well as the impact on whole body energy homeostasis. We describe immunodetection using whole-mount staining with freshly fixed tissues and paraffin-embedded tissue samples for the detection of microvessels in adipose tissue.

Original languageEnglish
Title of host publicationHandbook of Vascular Biology Techniques
PublisherSpringer Netherlands
Pages209-217
Number of pages9
ISBN (Print)9789401797160, 9789401797153
DOIs
Publication statusPublished - Jan 1 2015

Keywords

  • Endothelial cell isolation
  • Microvascular
  • Purification
  • Vascular pathology

ASJC Scopus subject areas

  • Medicine(all)
  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Fingerprint Dive into the research topics of 'Isolation and expansion of brain microvascular endothelial cells'. Together they form a unique fingerprint.

Cite this