Isolation and expansion of brain microvascular endothelial cells

Stefania Elena Navone; Giovanni Marfia; Giulio Alessandri

doi:10.1007/978-94-017-9716-0_18

Isolation and expansion of brain microvascular endothelial cells

Stefania Elena Navone, Giovanni Marfia, Giulio Alessandri

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

Here, we describe immunohistochemical methods to stain murine adipose tissue to study the structure of adipose vasculature and angiogenesis. This chapter describes an n vivo model to study adipose angiogenesis in mice. As opposed to in vitro angiogenesis assays, the described model recapitulates vascular alterations as well as the impact on whole body energy homeostasis. We describe immunodetection using whole-mount staining with freshly fixed tissues and paraffin-embedded tissue samples for the detection of microvessels in adipose tissue.

Original language	English
Title of host publication	Handbook of Vascular Biology Techniques
Publisher	Springer Netherlands
Pages	209-217
Number of pages	9
ISBN (Print)	9789401797160, 9789401797153
DOIs	https://doi.org/10.1007/978-94-017-9716-0_18
Publication status	Published - Jan 1 2015

Keywords

Endothelial cell isolation
Microvascular
Purification
Vascular pathology

ASJC Scopus subject areas

Medicine(all)
Agricultural and Biological Sciences(all)
Biochemistry, Genetics and Molecular Biology(all)

Access to Document

10.1007/978-94-017-9716-0_18

Cite this

@inbook{0d2d2dbddc6746f386cf0930b871c2f3,

title = "Isolation and expansion of brain microvascular endothelial cells",

abstract = "Here, we describe immunohistochemical methods to stain murine adipose tissue to study the structure of adipose vasculature and angiogenesis. This chapter describes an n vivo model to study adipose angiogenesis in mice. As opposed to in vitro angiogenesis assays, the described model recapitulates vascular alterations as well as the impact on whole body energy homeostasis. We describe immunodetection using whole-mount staining with freshly fixed tissues and paraffin-embedded tissue samples for the detection of microvessels in adipose tissue.",

keywords = "Endothelial cell isolation, Microvascular, Purification, Vascular pathology",

author = "Navone, {Stefania Elena} and Giovanni Marfia and Giulio Alessandri",

year = "2015",

month = jan,

day = "1",

doi = "10.1007/978-94-017-9716-0_18",

language = "English",

isbn = "9789401797160",

pages = "209--217",

booktitle = "Handbook of Vascular Biology Techniques",

publisher = "Springer Netherlands",

}

TY - CHAP

T1 - Isolation and expansion of brain microvascular endothelial cells

AU - Navone, Stefania Elena

AU - Marfia, Giovanni

AU - Alessandri, Giulio

PY - 2015/1/1

Y1 - 2015/1/1

N2 - Here, we describe immunohistochemical methods to stain murine adipose tissue to study the structure of adipose vasculature and angiogenesis. This chapter describes an n vivo model to study adipose angiogenesis in mice. As opposed to in vitro angiogenesis assays, the described model recapitulates vascular alterations as well as the impact on whole body energy homeostasis. We describe immunodetection using whole-mount staining with freshly fixed tissues and paraffin-embedded tissue samples for the detection of microvessels in adipose tissue.

AB - Here, we describe immunohistochemical methods to stain murine adipose tissue to study the structure of adipose vasculature and angiogenesis. This chapter describes an n vivo model to study adipose angiogenesis in mice. As opposed to in vitro angiogenesis assays, the described model recapitulates vascular alterations as well as the impact on whole body energy homeostasis. We describe immunodetection using whole-mount staining with freshly fixed tissues and paraffin-embedded tissue samples for the detection of microvessels in adipose tissue.

KW - Endothelial cell isolation

KW - Microvascular

KW - Purification

KW - Vascular pathology

UR - http://www.scopus.com/inward/record.url?scp=84943195755&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84943195755&partnerID=8YFLogxK

U2 - 10.1007/978-94-017-9716-0_18

DO - 10.1007/978-94-017-9716-0_18

M3 - Chapter

AN - SCOPUS:84943195755

SN - 9789401797160

SN - 9789401797153

SP - 209

EP - 217

BT - Handbook of Vascular Biology Techniques

PB - Springer Netherlands

ER -

Isolation and expansion of brain microvascular endothelial cells

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this