Isolation and expansion of brain microvascular endothelial cells

Stefania Elena Navone; Giovanni Marfia; Giulio Alessandri

doi:10.1007/978-94-017-9716-0_18

Isolation and expansion of brain microvascular endothelial cells

Stefania Elena Navone, Giovanni Marfia, Giulio Alessandri

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

Here, we describe immunohistochemical methods to stain murine adipose tissue to study the structure of adipose vasculature and angiogenesis. This chapter describes an n vivo model to study adipose angiogenesis in mice. As opposed to in vitro angiogenesis assays, the described model recapitulates vascular alterations as well as the impact on whole body energy homeostasis. We describe immunodetection using whole-mount staining with freshly fixed tissues and paraffin-embedded tissue samples for the detection of microvessels in adipose tissue.

Original language	English
Title of host publication	Handbook of Vascular Biology Techniques
Publisher	Springer Netherlands
Pages	209-217
Number of pages	9
ISBN (Print)	9789401797160, 9789401797153
DOIs	https://doi.org/10.1007/978-94-017-9716-0_18
Publication status	Published - Jan 1 2015

Keywords

Endothelial cell isolation
Microvascular
Purification
Vascular pathology

ASJC Scopus subject areas

Medicine(all)
Agricultural and Biological Sciences(all)
Biochemistry, Genetics and Molecular Biology(all)

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