Isolation and molecular characterization of Candida africana from Jos, Nigeria

Nnaemeka Emmanuel Nnadi; Grace Mebi Ayanbimpe; Fabio Scordino; Mark Ojogba Okolo; Ifeoma Bessy Enweani; Giuseppe Criseo; Orazio Romeo

doi:10.3109/13693786.2012.662598

Isolation and molecular characterization of Candida africana from Jos, Nigeria

Nnaemeka Emmanuel Nnadi, Grace Mebi Ayanbimpe, Fabio Scordino, Mark Ojogba Okolo, Ifeoma Bessy Enweani, Giuseppe Criseo, Orazio Romeo

IRCCS Centro Neurolesi "Bonino Pulejo"

Research output: Contribution to journal › Article

Abstract

During a survey of the prevalence of Candida spp. in Jos, Plateau State, Nigeria, two atypical C. albicans isolates were recovered. These two yeasts were germ tube positive, chlamydospore-negative and gave a green color on CHROMagar Candida. Molecular analysis performed by amplification of the hwp1 gene showed that these two isolates belonged to C. africana, a newly proposed Candida species closely related to C. albicans. Based on the presence or absence of an intron in DNA sequences encoding rRNA, the two C. africana, including all C. albicans isolates examined, were found to belong to genotype A and no other genotypes or species such as C. dubliniensis were found. To our knowledge, this is the first isolation of C. africana in Nigeria.

Original language	English
Pages (from-to)	765-767
Number of pages	3
Journal	Medical Mycology
Volume	50
Issue number	7
DOIs	https://doi.org/10.3109/13693786.2012.662598
Publication status	Published - Oct 2012

Keywords

Candida africana
Candida albicans
Candida dubliniensis
Chlamydospore-negative
Hwp1 gene

ASJC Scopus subject areas

veterinary(all)
Infectious Diseases

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title = "Isolation and molecular characterization of Candida africana from Jos, Nigeria",

abstract = "During a survey of the prevalence of Candida spp. in Jos, Plateau State, Nigeria, two atypical C. albicans isolates were recovered. These two yeasts were germ tube positive, chlamydospore-negative and gave a green color on CHROMagar Candida. Molecular analysis performed by amplification of the hwp1 gene showed that these two isolates belonged to C. africana, a newly proposed Candida species closely related to C. albicans. Based on the presence or absence of an intron in DNA sequences encoding rRNA, the two C. africana, including all C. albicans isolates examined, were found to belong to genotype A and no other genotypes or species such as C. dubliniensis were found. To our knowledge, this is the first isolation of C. africana in Nigeria.",

keywords = "Candida africana, Candida albicans, Candida dubliniensis, Chlamydospore-negative, Hwp1 gene",

author = "Nnadi, {Nnaemeka Emmanuel} and Ayanbimpe, {Grace Mebi} and Fabio Scordino and Okolo, {Mark Ojogba} and Enweani, {Ifeoma Bessy} and Giuseppe Criseo and Orazio Romeo",

year = "2012",

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language = "English",

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T1 - Isolation and molecular characterization of Candida africana from Jos, Nigeria

AU - Nnadi, Nnaemeka Emmanuel

AU - Ayanbimpe, Grace Mebi

AU - Scordino, Fabio

AU - Okolo, Mark Ojogba

AU - Enweani, Ifeoma Bessy

AU - Criseo, Giuseppe

AU - Romeo, Orazio

PY - 2012/10

Y1 - 2012/10

N2 - During a survey of the prevalence of Candida spp. in Jos, Plateau State, Nigeria, two atypical C. albicans isolates were recovered. These two yeasts were germ tube positive, chlamydospore-negative and gave a green color on CHROMagar Candida. Molecular analysis performed by amplification of the hwp1 gene showed that these two isolates belonged to C. africana, a newly proposed Candida species closely related to C. albicans. Based on the presence or absence of an intron in DNA sequences encoding rRNA, the two C. africana, including all C. albicans isolates examined, were found to belong to genotype A and no other genotypes or species such as C. dubliniensis were found. To our knowledge, this is the first isolation of C. africana in Nigeria.

AB - During a survey of the prevalence of Candida spp. in Jos, Plateau State, Nigeria, two atypical C. albicans isolates were recovered. These two yeasts were germ tube positive, chlamydospore-negative and gave a green color on CHROMagar Candida. Molecular analysis performed by amplification of the hwp1 gene showed that these two isolates belonged to C. africana, a newly proposed Candida species closely related to C. albicans. Based on the presence or absence of an intron in DNA sequences encoding rRNA, the two C. africana, including all C. albicans isolates examined, were found to belong to genotype A and no other genotypes or species such as C. dubliniensis were found. To our knowledge, this is the first isolation of C. africana in Nigeria.

KW - Candida africana

KW - Candida albicans

KW - Candida dubliniensis

KW - Chlamydospore-negative

KW - Hwp1 gene

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