Isolation and molecular cloning of a fast-growing strain of human hepatitis A virus from its double-stranded replicative form

A. Venuti, C. Di Russo, N. Del Grosso, A. M. Patti, F. Ruggeri, P. R. De Stasio, M. G. Martiniello, P. Pagnotti, A. M. Degener, M. Midulla

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Abstract

A fast-growing strain of human hepatitis A virus was selected and characterized. The virus has the unusual property of developing a strong cytopathic effect in tissue culture in 7 to 10 days. Sequences of the viral genome were cloned into recombinant plasmids with the double-stranded replicative form as a template for the reverse transcription of cDNA. Restriction analysis and direct sequencing indicate that this strain is different from that described by Ticehurst et al. in the region that presumptively codes for the major capsid protein VP1, but both isolates have conserved large areas of homology in the untranslated 5'-terminal sequences of the genome.

Original languageEnglish
Pages (from-to)579-588
Number of pages10
JournalJournal of Virology
Volume56
Issue number2
Publication statusPublished - 1985

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ASJC Scopus subject areas

  • Immunology

Cite this

Venuti, A., Di Russo, C., Del Grosso, N., Patti, A. M., Ruggeri, F., De Stasio, P. R., Martiniello, M. G., Pagnotti, P., Degener, A. M., & Midulla, M. (1985). Isolation and molecular cloning of a fast-growing strain of human hepatitis A virus from its double-stranded replicative form. Journal of Virology, 56(2), 579-588.