Isolation and preliminary characterization of an adriamycin-resistant murine fibrosarcoma cell line

R. Giavazzi, E. Scholar, I. R. Hart

Research output: Contribution to journalArticle

Abstract

A variant cell line (UV-2237-ADM(R)) resistant to the anthracycline antibiotic Adriamycin (doxorubicin) was selected in vitro from the murine UV-2237 fibrosarcoma tumor cell line. Resistance to Adriamycin proved to be a stable characteristic of the UV-2237-ADM(R) line, whether the line was grown in vivo or in vitro. The UV-2237-ADM(R) line also exhibited increased resistance to N-trifluoroacetyladriamycin-14-valerate, daunorubicin, actinomycin D, amsacrine, mitomycin C, vinblastine, and vincristine but not to bleomycin. Cell-cell hybridization studies showed that the Adriamycin resistance is an incompletely dominant trait. Uptake and efflux studies with [14C]Adriamycin indicated that the resistance exhibited by the UV-2237-ADM(R) line was due to both reduced uptake of the drug and an increased active efflux.

Original languageEnglish
Pages (from-to)2216-2222
Number of pages7
JournalCancer Research
Volume43
Issue number5
Publication statusPublished - 1983

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Fingerprint Dive into the research topics of 'Isolation and preliminary characterization of an adriamycin-resistant murine fibrosarcoma cell line'. Together they form a unique fingerprint.

  • Cite this