Isolation and preliminary characterization of an adriamycin-resistant murine fibrosarcoma cell line

R. Giavazzi, E. Scholar, I. R. Hart

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

A variant cell line (UV-2237-ADM(R)) resistant to the anthracycline antibiotic Adriamycin (doxorubicin) was selected in vitro from the murine UV-2237 fibrosarcoma tumor cell line. Resistance to Adriamycin proved to be a stable characteristic of the UV-2237-ADM(R) line, whether the line was grown in vivo or in vitro. The UV-2237-ADM(R) line also exhibited increased resistance to N-trifluoroacetyladriamycin-14-valerate, daunorubicin, actinomycin D, amsacrine, mitomycin C, vinblastine, and vincristine but not to bleomycin. Cell-cell hybridization studies showed that the Adriamycin resistance is an incompletely dominant trait. Uptake and efflux studies with [14C]Adriamycin indicated that the resistance exhibited by the UV-2237-ADM(R) line was due to both reduced uptake of the drug and an increased active efflux.

Original languageEnglish
Pages (from-to)2216-2222
Number of pages7
JournalCancer Research
Volume43
Issue number5
Publication statusPublished - 1983

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Fibrosarcoma
Doxorubicin
Cell Line
valrubicin
Amsacrine
Daunorubicin
Vinblastine
Anthracyclines
Bleomycin
Dactinomycin
Mitomycin
Vincristine
Tumor Cell Line
Anti-Bacterial Agents
Pharmaceutical Preparations
In Vitro Techniques

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Isolation and preliminary characterization of an adriamycin-resistant murine fibrosarcoma cell line. / Giavazzi, R.; Scholar, E.; Hart, I. R.

In: Cancer Research, Vol. 43, No. 5, 1983, p. 2216-2222.

Research output: Contribution to journalArticle

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