Isolation and structure of repressor-like proteins from the archaeon Sulfolobus solfataricus: Co-purification of RNase A with Sso7c

Udo C T Oppermann, Stefan Knapp, Valentina Bonetto, Rudolf Ladenstein, Hans Jörnvall

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The thermostable histone-like protein Sso7c (Sso for Sulfolobus solfataricus) from the archaeon Sulfolobus solfataricus was purified from the supernatant of acid-soluble cell lysates. Reverse phase HPLC of an apparently homogeneous Sso7c protein fraction from Mono S chromatography resulted in resolution of three further peaks. Sequence analysis revealed one of these components to be bovine RNase A, originating from the culture medium and explaining the RNA hydrolyzing activities of Sso7 preparations previously described. Sequence analysis of pure Sso7c showed an ε-Lys methylation pattern identical to that of Sso7d and a single Gln→Glu mutational difference at position 13. The remaining two proteins obtained after HPLC separation were identified as homologues of bacterial repressor-like proteins. Thus, the existence of repressor-like proteins was demonstrated at the protein level in archaea, raising the question of structural and functional consequences of these proteins on the otherwise eukaryotic-like basal transcriptional machinery in archaea. Copyright (C) 1998 Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)141-144
Number of pages4
JournalFEBS Letters
Volume432
Issue number3
DOIs
Publication statusPublished - Aug 7 1998

Fingerprint

Sulfolobus solfataricus
Repressor Proteins
Pancreatic Ribonuclease
Archaea
Purification
Sequence Analysis
Proteins
High Pressure Liquid Chromatography
Bacterial Proteins
Histones
Methylation
Culture Media
Chromatography
RNA
Acids
Machinery

Keywords

  • Archaeon
  • Repressor
  • Transcription

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Isolation and structure of repressor-like proteins from the archaeon Sulfolobus solfataricus : Co-purification of RNase A with Sso7c. / Oppermann, Udo C T; Knapp, Stefan; Bonetto, Valentina; Ladenstein, Rudolf; Jörnvall, Hans.

In: FEBS Letters, Vol. 432, No. 3, 07.08.1998, p. 141-144.

Research output: Contribution to journalArticle

Oppermann, Udo C T ; Knapp, Stefan ; Bonetto, Valentina ; Ladenstein, Rudolf ; Jörnvall, Hans. / Isolation and structure of repressor-like proteins from the archaeon Sulfolobus solfataricus : Co-purification of RNase A with Sso7c. In: FEBS Letters. 1998 ; Vol. 432, No. 3. pp. 141-144.
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