Isolation and sub-chromosomal localization of a DNA fragment of the human choline acetyltransferase gene

Riccardo Cervini, Mariano Rocchi, Stefano DiDonato, Gaetano Finocchiaro

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

A DNA fragment of 219 bp was obtained by polymerase chain reaction (PCR) on human genomic DNA using two oligonucleotide mixtures derived from peptide sequences of human placenta choline acetyltransferase (ChAT) and from partially conserved amino acid sequences of rat, porcine and Drosophila ChAT. Sequence homology with porcine ChAT demonstrated that this fragment is part of the human ChAT gene. This gene was assigned to chromosome 10 by hybridization of the 219 bp DNA probe with DNA from human-hamster somatic cell hybrids, and to region 10q 11.2-10qter by PCR experiments.

Original languageEnglish
Pages (from-to)191-194
Number of pages4
JournalNeuroscience Letters
Volume132
Issue number2
DOIs
Publication statusPublished - Nov 11 1991

Fingerprint

Choline O-Acetyltransferase
DNA
Genes
Swine
Polymerase Chain Reaction
Chromosomes, Human, Pair 10
Hybrid Cells
DNA Probes
Sequence Homology
Oligonucleotides
Cricetinae
Placenta
Drosophila
Amino Acid Sequence
Peptides

Keywords

  • Choline acetyltransferase
  • Gene mapping
  • Polymerase chain reaction

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Isolation and sub-chromosomal localization of a DNA fragment of the human choline acetyltransferase gene. / Cervini, Riccardo; Rocchi, Mariano; DiDonato, Stefano; Finocchiaro, Gaetano.

In: Neuroscience Letters, Vol. 132, No. 2, 11.11.1991, p. 191-194.

Research output: Contribution to journalArticle

Cervini, Riccardo ; Rocchi, Mariano ; DiDonato, Stefano ; Finocchiaro, Gaetano. / Isolation and sub-chromosomal localization of a DNA fragment of the human choline acetyltransferase gene. In: Neuroscience Letters. 1991 ; Vol. 132, No. 2. pp. 191-194.
@article{d9f20e247006473a8d3bf4f2c4178e71,
title = "Isolation and sub-chromosomal localization of a DNA fragment of the human choline acetyltransferase gene",
abstract = "A DNA fragment of 219 bp was obtained by polymerase chain reaction (PCR) on human genomic DNA using two oligonucleotide mixtures derived from peptide sequences of human placenta choline acetyltransferase (ChAT) and from partially conserved amino acid sequences of rat, porcine and Drosophila ChAT. Sequence homology with porcine ChAT demonstrated that this fragment is part of the human ChAT gene. This gene was assigned to chromosome 10 by hybridization of the 219 bp DNA probe with DNA from human-hamster somatic cell hybrids, and to region 10q 11.2-10qter by PCR experiments.",
keywords = "Choline acetyltransferase, Gene mapping, Polymerase chain reaction",
author = "Riccardo Cervini and Mariano Rocchi and Stefano DiDonato and Gaetano Finocchiaro",
year = "1991",
month = "11",
day = "11",
doi = "10.1016/0304-3940(91)90299-9",
language = "English",
volume = "132",
pages = "191--194",
journal = "Neuroscience Letters",
issn = "0304-3940",
publisher = "Elsevier Ireland Ltd",
number = "2",

}

TY - JOUR

T1 - Isolation and sub-chromosomal localization of a DNA fragment of the human choline acetyltransferase gene

AU - Cervini, Riccardo

AU - Rocchi, Mariano

AU - DiDonato, Stefano

AU - Finocchiaro, Gaetano

PY - 1991/11/11

Y1 - 1991/11/11

N2 - A DNA fragment of 219 bp was obtained by polymerase chain reaction (PCR) on human genomic DNA using two oligonucleotide mixtures derived from peptide sequences of human placenta choline acetyltransferase (ChAT) and from partially conserved amino acid sequences of rat, porcine and Drosophila ChAT. Sequence homology with porcine ChAT demonstrated that this fragment is part of the human ChAT gene. This gene was assigned to chromosome 10 by hybridization of the 219 bp DNA probe with DNA from human-hamster somatic cell hybrids, and to region 10q 11.2-10qter by PCR experiments.

AB - A DNA fragment of 219 bp was obtained by polymerase chain reaction (PCR) on human genomic DNA using two oligonucleotide mixtures derived from peptide sequences of human placenta choline acetyltransferase (ChAT) and from partially conserved amino acid sequences of rat, porcine and Drosophila ChAT. Sequence homology with porcine ChAT demonstrated that this fragment is part of the human ChAT gene. This gene was assigned to chromosome 10 by hybridization of the 219 bp DNA probe with DNA from human-hamster somatic cell hybrids, and to region 10q 11.2-10qter by PCR experiments.

KW - Choline acetyltransferase

KW - Gene mapping

KW - Polymerase chain reaction

UR - http://www.scopus.com/inward/record.url?scp=0026072269&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026072269&partnerID=8YFLogxK

U2 - 10.1016/0304-3940(91)90299-9

DO - 10.1016/0304-3940(91)90299-9

M3 - Article

C2 - 1784419

AN - SCOPUS:0026072269

VL - 132

SP - 191

EP - 194

JO - Neuroscience Letters

JF - Neuroscience Letters

SN - 0304-3940

IS - 2

ER -