Isolation and sub-chromosomal localization of a DNA fragment of the human choline acetyltransferase gene

Riccardo Cervini, Mariano Rocchi, Stefano DiDonato, Gaetano Finocchiaro

Research output: Contribution to journalArticle

Abstract

A DNA fragment of 219 bp was obtained by polymerase chain reaction (PCR) on human genomic DNA using two oligonucleotide mixtures derived from peptide sequences of human placenta choline acetyltransferase (ChAT) and from partially conserved amino acid sequences of rat, porcine and Drosophila ChAT. Sequence homology with porcine ChAT demonstrated that this fragment is part of the human ChAT gene. This gene was assigned to chromosome 10 by hybridization of the 219 bp DNA probe with DNA from human-hamster somatic cell hybrids, and to region 10q 11.2-10qter by PCR experiments.

Original languageEnglish
Pages (from-to)191-194
Number of pages4
JournalNeuroscience Letters
Volume132
Issue number2
DOIs
Publication statusPublished - Nov 11 1991

Keywords

  • Choline acetyltransferase
  • Gene mapping
  • Polymerase chain reaction

ASJC Scopus subject areas

  • Neuroscience(all)

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