Isolation, characterization, and differentiation of multipotent neural progenitor cells from human cerebrospinal fluid in fetal cystic myelomeningocele

Mario Marotta, Alejandra Fernández-Martín, Marc Oria, Cesar G. Fontecha, Carles Giné, Vicente Martínez-Ibáñez, Elena Carreras, Michael A. Belfort, Gloria Pelizzo, Jose L. Peiró

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Despite benefits of prenatal in utero repair of myelomeningocele, a severe type of spina bifida aperta, many of these patients will still suffer mild to severe impairment. One potential source of stem cells for new regenerative medicine-based therapeutic approaches for spinal cord injury repair is neural progenitor cells (NPCs) in cerebrospinal fluid (CSF). To this aim, we extracted CSF from the cyst surrounding the exposed neural placode during the surgical repair of myelomeningocele in 6 fetuses (20 to 26 weeks of gestation). In primary cultured CSF-derived cells, neurogenic properties were confirmed by in vitro differentiation into various neural lineage cell types, and NPC markers expression (TBR2, CD15, SOX2) were detected by immunofluorescence and RT-PCR analysis. Differentiation into three neural lineages was corroborated by arbitrary differentiation (depletion of growths factors) or explicit differentiation as neuronal, astrocyte, or oligodendrocyte cell types using specific induction mediums. Differentiated cells showed the specific expression of neural differentiation markers (βIII-tubulin, GFAP, CNPase, oligo-O1). In myelomeningocele patients, CSF-derived cells could become a potential source of NPCs with neurogenic capacity. Our findings support the development of innovative stem-cell-based therapeutics by autologous transplantation of CSF-derived NPCs in damaged spinal cords, such as myelomeningocele, thus promoting neural tissue regeneration in fetuses.

Original languageEnglish
Pages (from-to)33-42
Number of pages10
JournalStem Cell Research
Volume22
DOIs
Publication statusPublished - Jul 1 2017

Fingerprint

Meningomyelocele
Cerebrospinal Fluid
Stem Cells
2',3'-Cyclic-Nucleotide Phosphodiesterases
Fetus
Spina Bifida Cystica
Spinal Cord Regeneration
Growth Differentiation Factors
Regenerative Medicine
Autologous Transplantation
Differentiation Antigens
Oligodendroglia
Tubulin
Spinal Cord Injuries
Astrocytes
Fluorescent Antibody Technique
Cysts
Regeneration
Spinal Cord
Pregnancy

Keywords

  • Cerebrospinal fluid
  • Fetal therapy
  • Human primary cell cultures
  • Myelomeningocele
  • Neural differentiation
  • Neural precursor cells

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

Cite this

Marotta, M., Fernández-Martín, A., Oria, M., Fontecha, C. G., Giné, C., Martínez-Ibáñez, V., ... Peiró, J. L. (2017). Isolation, characterization, and differentiation of multipotent neural progenitor cells from human cerebrospinal fluid in fetal cystic myelomeningocele. Stem Cell Research, 22, 33-42. https://doi.org/10.1016/j.scr.2017.05.003

Isolation, characterization, and differentiation of multipotent neural progenitor cells from human cerebrospinal fluid in fetal cystic myelomeningocele. / Marotta, Mario; Fernández-Martín, Alejandra; Oria, Marc; Fontecha, Cesar G.; Giné, Carles; Martínez-Ibáñez, Vicente; Carreras, Elena; Belfort, Michael A.; Pelizzo, Gloria; Peiró, Jose L.

In: Stem Cell Research, Vol. 22, 01.07.2017, p. 33-42.

Research output: Contribution to journalArticle

Marotta, M, Fernández-Martín, A, Oria, M, Fontecha, CG, Giné, C, Martínez-Ibáñez, V, Carreras, E, Belfort, MA, Pelizzo, G & Peiró, JL 2017, 'Isolation, characterization, and differentiation of multipotent neural progenitor cells from human cerebrospinal fluid in fetal cystic myelomeningocele', Stem Cell Research, vol. 22, pp. 33-42. https://doi.org/10.1016/j.scr.2017.05.003
Marotta, Mario ; Fernández-Martín, Alejandra ; Oria, Marc ; Fontecha, Cesar G. ; Giné, Carles ; Martínez-Ibáñez, Vicente ; Carreras, Elena ; Belfort, Michael A. ; Pelizzo, Gloria ; Peiró, Jose L. / Isolation, characterization, and differentiation of multipotent neural progenitor cells from human cerebrospinal fluid in fetal cystic myelomeningocele. In: Stem Cell Research. 2017 ; Vol. 22. pp. 33-42.
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