TY - JOUR
T1 - Isolation, characterization and ex vivo expansion of CD34+ cells from umbilical cord blood
AU - Pasino, Mirella
AU - Biglino, Paola
AU - Corcione, Anna
AU - Scuderi, Francesca
AU - Franchini, Edoardo
AU - Tasso, Paola
AU - De Biasio, Pierangela
AU - Marotta, Fernando
AU - Valetto, Angelo
AU - Pistoia, Vito
AU - Mori, Pier Giorgio
PY - 1998
Y1 - 1998
N2 - Problem. Umbilical cord blood (UCB) represents an alternative to bone marrow or peripheral blood as a source of hemopoietic progenitors that can be transplanted in an autologous or allogeneic setting, particularly in children. Methods. UCB CD34+ cells were purified by magnetic cell sorting, analyzed by flow cytometry, tested for proliferative response and clonogenic capability in the presence of SCF+IL-6 (CSF2) or SCF+IL-6 + IL-3 + GM-CSF + G-CSF + EPO (CSF6). Results. Median CD34+ yield was 84.5%, median CD34+ population purity was 96%. The starting CD34+ population, that was predominantly CD38+, HLA-DR+ and CD33+, expanded 11-15 fold by CSF2 and 35-67 fold by CSF6 stimulation. The CD34+CD33+ and CD34-CD33+ subsets expanded more in the presence of the CSF2 than of the CSF6 combination. CSF6 was more efficient than CSF2 in increasing CFU-GM, CFU-E and/or BFU-E. Conclusions. These data suggest that a selective amplification of different types of hemopoietic progenitors may be obtained by varying cytokine combinations.
AB - Problem. Umbilical cord blood (UCB) represents an alternative to bone marrow or peripheral blood as a source of hemopoietic progenitors that can be transplanted in an autologous or allogeneic setting, particularly in children. Methods. UCB CD34+ cells were purified by magnetic cell sorting, analyzed by flow cytometry, tested for proliferative response and clonogenic capability in the presence of SCF+IL-6 (CSF2) or SCF+IL-6 + IL-3 + GM-CSF + G-CSF + EPO (CSF6). Results. Median CD34+ yield was 84.5%, median CD34+ population purity was 96%. The starting CD34+ population, that was predominantly CD38+, HLA-DR+ and CD33+, expanded 11-15 fold by CSF2 and 35-67 fold by CSF6 stimulation. The CD34+CD33+ and CD34-CD33+ subsets expanded more in the presence of the CSF2 than of the CSF6 combination. CSF6 was more efficient than CSF2 in increasing CFU-GM, CFU-E and/or BFU-E. Conclusions. These data suggest that a selective amplification of different types of hemopoietic progenitors may be obtained by varying cytokine combinations.
KW - Cytokines
KW - Ex vivo expansion
KW - Hemopoietic progenitors
KW - Immunophenotype
KW - Umbilical cord blood
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M3 - Article
AN - SCOPUS:0031761276
VL - 5
SP - 151
EP - 159
JO - Cancer Research, Therapy and Control
JF - Cancer Research, Therapy and Control
SN - 1064-0525
IS - 3
ER -