Isolation, characterization and ex vivo expansion of CD34+ cells from umbilical cord blood

Mirella Pasino, Paola Biglino, Anna Corcione, Francesca Scuderi, Edoardo Franchini, Paola Tasso, Pierangela De Biasio, Fernando Marotta, Angelo Valetto, Vito Pistoia, Pier Giorgio Mori

Research output: Contribution to journal › Article

Abstract

Problem. Umbilical cord blood (UCB) represents an alternative to bone marrow or peripheral blood as a source of hemopoietic progenitors that can be transplanted in an autologous or allogeneic setting, particularly in children. Methods. UCB CD34+ cells were purified by magnetic cell sorting, analyzed by flow cytometry, tested for proliferative response and clonogenic capability in the presence of SCF+IL-6 (CSF2) or SCF+IL-6 + IL-3 + GM-CSF + G-CSF + EPO (CSF6). Results. Median CD34+ yield was 84.5%, median CD34+ population purity was 96%. The starting CD34+ population, that was predominantly CD38+, HLA-DR+ and CD33+, expanded 11-15 fold by CSF2 and 35-67 fold by CSF6 stimulation. The CD34+CD33+ and CD34-CD33+ subsets expanded more in the presence of the CSF2 than of the CSF6 combination. CSF6 was more efficient than CSF2 in increasing CFU-GM, CFU-E and/or BFU-E. Conclusions. These data suggest that a selective amplification of different types of hemopoietic progenitors may be obtained by varying cytokine combinations.

Original languageEnglish
Pages (from-to)151-159
Number of pages9
JournalCancer Research Therapy and Control
Volume5
Issue number3
Publication statusPublished - 1998

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Granulocyte-Macrophage Progenitor Cells
Erythroid Precursor Cells
Fetal Blood
Interleukin-6
Interleukin-3
HLA-DR Antigens
Granulocyte Colony-Stimulating Factor
Granulocyte-Macrophage Colony-Stimulating Factor
Population
Blood Cells
Flow Cytometry
Bone Marrow
Cytokines

Keywords

  • Cytokines
  • Ex vivo expansion
  • Hemopoietic progenitors
  • Immunophenotype
  • Umbilical cord blood

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Pasino, M., Biglino, P., Corcione, A., Scuderi, F., Franchini, E., Tasso, P., ... Mori, P. G. (1998). Isolation, characterization and ex vivo expansion of CD34+ cells from umbilical cord blood. Cancer Research Therapy and Control, 5(3), 151-159.

Isolation, characterization and ex vivo expansion of CD34+ cells from umbilical cord blood. / Pasino, Mirella; Biglino, Paola; Corcione, Anna; Scuderi, Francesca; Franchini, Edoardo; Tasso, Paola; De Biasio, Pierangela; Marotta, Fernando; Valetto, Angelo; Pistoia, Vito; Mori, Pier Giorgio.

In: Cancer Research Therapy and Control, Vol. 5, No. 3, 1998, p. 151-159.

Research output: Contribution to journal › Article

Pasino, M, Biglino, P, Corcione, A, Scuderi, F, Franchini, E, Tasso, P, De Biasio, P, Marotta, F, Valetto, A, Pistoia, V & Mori, PG 1998, 'Isolation, characterization and ex vivo expansion of CD34+ cells from umbilical cord blood', Cancer Research Therapy and Control, vol. 5, no. 3, pp. 151-159.
Pasino M, Biglino P, Corcione A, Scuderi F, Franchini E, Tasso P et al. Isolation, characterization and ex vivo expansion of CD34+ cells from umbilical cord blood. Cancer Research Therapy and Control. 1998;5(3):151-159.
Pasino, Mirella ; Biglino, Paola ; Corcione, Anna ; Scuderi, Francesca ; Franchini, Edoardo ; Tasso, Paola ; De Biasio, Pierangela ; Marotta, Fernando ; Valetto, Angelo ; Pistoia, Vito ; Mori, Pier Giorgio. / Isolation, characterization and ex vivo expansion of CD34+ cells from umbilical cord blood. In: Cancer Research Therapy and Control. 1998 ; Vol. 5, No. 3. pp. 151-159.
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AU - Pasino, Mirella

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AU - Franchini, Edoardo

AU - Tasso, Paola

AU - De Biasio, Pierangela

AU - Marotta, Fernando

AU - Valetto, Angelo

AU - Pistoia, Vito

AU - Mori, Pier Giorgio

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N2 - Problem. Umbilical cord blood (UCB) represents an alternative to bone marrow or peripheral blood as a source of hemopoietic progenitors that can be transplanted in an autologous or allogeneic setting, particularly in children. Methods. UCB CD34+ cells were purified by magnetic cell sorting, analyzed by flow cytometry, tested for proliferative response and clonogenic capability in the presence of SCF+IL-6 (CSF2) or SCF+IL-6 + IL-3 + GM-CSF + G-CSF + EPO (CSF6). Results. Median CD34+ yield was 84.5%, median CD34+ population purity was 96%. The starting CD34+ population, that was predominantly CD38+, HLA-DR+ and CD33+, expanded 11-15 fold by CSF2 and 35-67 fold by CSF6 stimulation. The CD34+CD33+ and CD34-CD33+ subsets expanded more in the presence of the CSF2 than of the CSF6 combination. CSF6 was more efficient than CSF2 in increasing CFU-GM, CFU-E and/or BFU-E. Conclusions. These data suggest that a selective amplification of different types of hemopoietic progenitors may be obtained by varying cytokine combinations.

AB - Problem. Umbilical cord blood (UCB) represents an alternative to bone marrow or peripheral blood as a source of hemopoietic progenitors that can be transplanted in an autologous or allogeneic setting, particularly in children. Methods. UCB CD34+ cells were purified by magnetic cell sorting, analyzed by flow cytometry, tested for proliferative response and clonogenic capability in the presence of SCF+IL-6 (CSF2) or SCF+IL-6 + IL-3 + GM-CSF + G-CSF + EPO (CSF6). Results. Median CD34+ yield was 84.5%, median CD34+ population purity was 96%. The starting CD34+ population, that was predominantly CD38+, HLA-DR+ and CD33+, expanded 11-15 fold by CSF2 and 35-67 fold by CSF6 stimulation. The CD34+CD33+ and CD34-CD33+ subsets expanded more in the presence of the CSF2 than of the CSF6 combination. CSF6 was more efficient than CSF2 in increasing CFU-GM, CFU-E and/or BFU-E. Conclusions. These data suggest that a selective amplification of different types of hemopoietic progenitors may be obtained by varying cytokine combinations.

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