Isolation, characterization, and transduction of endometrial decidual tissue multipotent mesenchymal stromal/stem cells from menstrual blood

Filippo Rossignoli, Anna Caselli, Giulia Grisendi, Serena Piccinno, Jorge S. Burns, Alba Murgia, Elena Veronesi, Pietro Loschi, Cristina Masini, Pierfranco Conte, Paolo Paolucci, Edwin M. Horwiz, Massimo Dominici

Research output: Contribution to journalArticle

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Abstract

Mesenchymal stromal/stem cells (MSCs) reveal progenitor cells-like features including proliferation and differentiation capacities. One of the most historically recognized sources of MSC has been the bone marrow, while other sources recently include adipose tissue, teeth, bone, muscle, placenta, liver, pancreas, umbilical cord, and cord blood. Frequently, progenitor isolation requires traumatic procedures that are poorly feasible and associated with patient discomfort. In the attempt to identify a more approachable MSC source, we focused on endometrial decidual tissue (EDT) found within menstrual blood. Based also on recent literature findings, we hypothesized that EDT may contain heterogeneous populations including some having MSC-like features. Thus, we here sought to isolate EDT-MSC processing menstrual samples from multiple donors. Cytofluorimetric analyses revealed that resulting adherent cells were expressing mesenchymal surface markers, including CD56, CD73, CD90, CD105 and CD146, and pluripotency markers such as SSEA-4. Moreover, EDT-MSC showed a robust clonogenic potential and could be largely expanded in vitro as fibroblastoid elements. In addition, differentiation assays drove these cells towards osteogenic, adipogenic, and chondrogenic lineages. Finally, for the first time, we were able to gene modify these progenitors by a retroviral vector carrying the green fluorescent protein. From these data, we suggest that EDT-MSC could represent a new promising tool having potential within cell and gene therapy applications.

Original languageEnglish
Article number901821
JournalBioMed Research International
Volume2013
DOIs
Publication statusPublished - 2013

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Multipotent Stem Cells
Stem cells
Mesenchymal Stromal Cells
Blood
Tissue
Bone
Gene therapy
Green Fluorescent Proteins
Liver
Muscle
Assays
Genes
Umbilical Cord
Cell- and Tissue-Based Therapy
Processing
Fetal Blood

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Isolation, characterization, and transduction of endometrial decidual tissue multipotent mesenchymal stromal/stem cells from menstrual blood. / Rossignoli, Filippo; Caselli, Anna; Grisendi, Giulia; Piccinno, Serena; Burns, Jorge S.; Murgia, Alba; Veronesi, Elena; Loschi, Pietro; Masini, Cristina; Conte, Pierfranco; Paolucci, Paolo; Horwiz, Edwin M.; Dominici, Massimo.

In: BioMed Research International, Vol. 2013, 901821, 2013.

Research output: Contribution to journalArticle

Rossignoli, Filippo ; Caselli, Anna ; Grisendi, Giulia ; Piccinno, Serena ; Burns, Jorge S. ; Murgia, Alba ; Veronesi, Elena ; Loschi, Pietro ; Masini, Cristina ; Conte, Pierfranco ; Paolucci, Paolo ; Horwiz, Edwin M. ; Dominici, Massimo. / Isolation, characterization, and transduction of endometrial decidual tissue multipotent mesenchymal stromal/stem cells from menstrual blood. In: BioMed Research International. 2013 ; Vol. 2013.
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