Isolation, Expansion, and Characterization of Natural Killer Cells and Their Precursors as a Tool to Study Cancer Immunosurveillance

Monica Parodi, Raffaella Meazza, Chiara Vitale, Gabriella Pietra, Paolo Carrega, Massimo Vitale

Research output: Contribution to journal › Article

Abstract

This chapter will describe the current methodologies to isolate and expand NK cells from Peripheral Blood (PB) or tissues for "in vitro" studies, including NK cell antitumor immune function. In addition, methods to induce NK cell maturation, differentiation, and expansion from CD34+ precursors will also be described. Finally, it will also be treated the topical issue of the characterization of new functionally and phenotypically defined NK cell subsets.

LanguageEnglish
Pages87-117
Number of pages31
JournalMethods in molecular biology (Clifton, N.J.)
Volume1884
DOIs
Publication statusPublished - Jan 1 2019

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Immunologic Monitoring
Natural Killer Cells
Neoplasms
Cell Differentiation

Keywords

  • Cell isolation
  • Chemotaxis
  • Cytofluorimetric analysis
  • Cytokines
  • Cytotoxicity
  • Differentiation
  • Maturation
  • Migration
  • Natural Killer cells
  • Precursors
  • Tissues

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Isolation, Expansion, and Characterization of Natural Killer Cells and Their Precursors as a Tool to Study Cancer Immunosurveillance. / Parodi, Monica; Meazza, Raffaella; Vitale, Chiara; Pietra, Gabriella; Carrega, Paolo; Vitale, Massimo.

In: Methods in molecular biology (Clifton, N.J.), Vol. 1884, 01.01.2019, p. 87-117.

Research output: Contribution to journal › Article

Parodi, M, Meazza, R, Vitale, C, Pietra, G, Carrega, P & Vitale, M 2019, 'Isolation, Expansion, and Characterization of Natural Killer Cells and Their Precursors as a Tool to Study Cancer Immunosurveillance', Methods in molecular biology (Clifton, N.J.), vol. 1884, pp. 87-117. https://doi.org/10.1007/978-1-4939-8885-3_6
Parodi M, Meazza R, Vitale C, Pietra G, Carrega P, Vitale M. Isolation, Expansion, and Characterization of Natural Killer Cells and Their Precursors as a Tool to Study Cancer Immunosurveillance. Methods in molecular biology (Clifton, N.J.). 2019 Jan 1;1884:87-117. https://doi.org/10.1007/978-1-4939-8885-3_6
Parodi, Monica ; Meazza, Raffaella ; Vitale, Chiara ; Pietra, Gabriella ; Carrega, Paolo ; Vitale, Massimo. / Isolation, Expansion, and Characterization of Natural Killer Cells and Their Precursors as a Tool to Study Cancer Immunosurveillance. In: Methods in molecular biology (Clifton, N.J.). 2019 ; Vol. 1884. pp. 87-117.
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