Isolation, Expansion, and Characterization of Natural Killer Cells and Their Precursors as a Tool to Study Cancer Immunosurveillance

Monica Parodi; Raffaella Meazza; Chiara Vitale; Gabriella Pietra; Paolo Carrega; Massimo Vitale

doi:10.1007/978-1-4939-8885-3_6

Isolation, Expansion, and Characterization of Natural Killer Cells and Their Precursors as a Tool to Study Cancer Immunosurveillance

Monica Parodi, Raffaella Meazza, Chiara Vitale, Gabriella Pietra, Paolo Carrega, Massimo Vitale

Research output: Contribution to journal › Article › peer-review

Abstract

This chapter will describe the current methodologies to isolate and expand NK cells from Peripheral Blood (PB) or tissues for "in vitro" studies, including NK cell antitumor immune function. In addition, methods to induce NK cell maturation, differentiation, and expansion from CD34+ precursors will also be described. Finally, it will also be treated the topical issue of the characterization of new functionally and phenotypically defined NK cell subsets.

Original language	English
Pages (from-to)	87-117
Number of pages	31
Journal	Methods in molecular biology (Clifton, N.J.)
Volume	1884
DOIs	https://doi.org/10.1007/978-1-4939-8885-3_6
Publication status	Published - Jan 1 2019

Keywords

Cell isolation
Chemotaxis
Cytofluorimetric analysis
Cytokines
Cytotoxicity
Differentiation
Maturation
Migration
Natural Killer cells
Precursors
Tissues

ASJC Scopus subject areas

Molecular Biology
Genetics

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10.1007/978-1-4939-8885-3_6

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Isolation, Expansion, and Characterization of Natural Killer Cells and Their Precursors as a Tool to Study Cancer Immunosurveillance. / Parodi, Monica; Meazza, Raffaella ; Vitale, Chiara ; Pietra, Gabriella; Carrega, Paolo; Vitale, Massimo.

In: Methods in molecular biology (Clifton, N.J.), Vol. 1884, 01.01.2019, p. 87-117.

Research output: Contribution to journal › Article › peer-review

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AU - Carrega, Paolo

AU - Vitale, Massimo

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Isolation, Expansion, and Characterization of Natural Killer Cells and Their Precursors as a Tool to Study Cancer Immunosurveillance

Abstract

Keywords

ASJC Scopus subject areas

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