Abstract
This chapter will describe the current methodologies to isolate and expand NK cells from Peripheral Blood (PB) or tissues for "in vitro" studies, including NK cell antitumor immune function. In addition, methods to induce NK cell maturation, differentiation, and expansion from CD34+ precursors will also be described. Finally, it will also be treated the topical issue of the characterization of new functionally and phenotypically defined NK cell subsets.
Original language | English |
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Pages (from-to) | 87-117 |
Number of pages | 31 |
Journal | Methods in molecular biology (Clifton, N.J.) |
Volume | 1884 |
DOIs | |
Publication status | Published - Jan 1 2019 |
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Keywords
- Cell isolation
- Chemotaxis
- Cytofluorimetric analysis
- Cytokines
- Cytotoxicity
- Differentiation
- Maturation
- Migration
- Natural Killer cells
- Precursors
- Tissues
ASJC Scopus subject areas
- Molecular Biology
- Genetics
Cite this
Isolation, Expansion, and Characterization of Natural Killer Cells and Their Precursors as a Tool to Study Cancer Immunosurveillance. / Parodi, Monica; Meazza, Raffaella; Vitale, Chiara; Pietra, Gabriella; Carrega, Paolo; Vitale, Massimo.
In: Methods in molecular biology (Clifton, N.J.), Vol. 1884, 01.01.2019, p. 87-117.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Isolation, Expansion, and Characterization of Natural Killer Cells and Their Precursors as a Tool to Study Cancer Immunosurveillance
AU - Parodi, Monica
AU - Meazza, Raffaella
AU - Vitale, Chiara
AU - Pietra, Gabriella
AU - Carrega, Paolo
AU - Vitale, Massimo
PY - 2019/1/1
Y1 - 2019/1/1
N2 - This chapter will describe the current methodologies to isolate and expand NK cells from Peripheral Blood (PB) or tissues for "in vitro" studies, including NK cell antitumor immune function. In addition, methods to induce NK cell maturation, differentiation, and expansion from CD34+ precursors will also be described. Finally, it will also be treated the topical issue of the characterization of new functionally and phenotypically defined NK cell subsets.
AB - This chapter will describe the current methodologies to isolate and expand NK cells from Peripheral Blood (PB) or tissues for "in vitro" studies, including NK cell antitumor immune function. In addition, methods to induce NK cell maturation, differentiation, and expansion from CD34+ precursors will also be described. Finally, it will also be treated the topical issue of the characterization of new functionally and phenotypically defined NK cell subsets.
KW - Cell isolation
KW - Chemotaxis
KW - Cytofluorimetric analysis
KW - Cytokines
KW - Cytotoxicity
KW - Differentiation
KW - Maturation
KW - Migration
KW - Natural Killer cells
KW - Precursors
KW - Tissues
UR - http://www.scopus.com/inward/record.url?scp=85056975054&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85056975054&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-8885-3_6
DO - 10.1007/978-1-4939-8885-3_6
M3 - Article
C2 - 30465197
AN - SCOPUS:85056975054
VL - 1884
SP - 87
EP - 117
JO - Methods in Molecular Biology
JF - Methods in Molecular Biology
SN - 1064-3745
ER -