Isolation, Expansion, and Characterization of Natural Killer Cells and Their Precursors as a Tool to Study Cancer Immunosurveillance

Monica Parodi; Raffaella Meazza; Chiara Vitale; Gabriella Pietra; Paolo Carrega; Massimo Vitale

doi:10.1007/978-1-4939-8885-3_6

Isolation, Expansion, and Characterization of Natural Killer Cells and Their Precursors as a Tool to Study Cancer Immunosurveillance

Monica Parodi, Raffaella Meazza, Chiara Vitale, Gabriella Pietra, Paolo Carrega, Massimo Vitale

Research output: Contribution to journal › Article › peer-review

Abstract

This chapter will describe the current methodologies to isolate and expand NK cells from Peripheral Blood (PB) or tissues for "in vitro" studies, including NK cell antitumor immune function. In addition, methods to induce NK cell maturation, differentiation, and expansion from CD34+ precursors will also be described. Finally, it will also be treated the topical issue of the characterization of new functionally and phenotypically defined NK cell subsets.

Original language	English
Pages (from-to)	87-117
Number of pages	31
Journal	Methods in molecular biology (Clifton, N.J.)
Volume	1884
DOIs	https://doi.org/10.1007/978-1-4939-8885-3_6
Publication status	Published - Jan 1 2019

Keywords

Cell isolation
Chemotaxis
Cytofluorimetric analysis
Cytokines
Cytotoxicity
Differentiation
Maturation
Migration
Natural Killer cells
Precursors
Tissues

ASJC Scopus subject areas

Molecular Biology
Genetics

Access to Document

10.1007/978-1-4939-8885-3_6

Cite this

Isolation, Expansion, and Characterization of Natural Killer Cells and Their Precursors as a Tool to Study Cancer Immunosurveillance. / Parodi, Monica; Meazza, Raffaella ; Vitale, Chiara ; Pietra, Gabriella; Carrega, Paolo; Vitale, Massimo.

In: Methods in molecular biology (Clifton, N.J.), Vol. 1884, 01.01.2019, p. 87-117.

Research output: Contribution to journal › Article › peer-review

@article{55ff058ae67e408d97a6f15ca05d6047,

title = "Isolation, Expansion, and Characterization of Natural Killer Cells and Their Precursors as a Tool to Study Cancer Immunosurveillance",

abstract = "This chapter will describe the current methodologies to isolate and expand NK cells from Peripheral Blood (PB) or tissues for {"}in vitro{"} studies, including NK cell antitumor immune function. In addition, methods to induce NK cell maturation, differentiation, and expansion from CD34+ precursors will also be described. Finally, it will also be treated the topical issue of the characterization of new functionally and phenotypically defined NK cell subsets.",

keywords = "Cell isolation, Chemotaxis, Cytofluorimetric analysis, Cytokines, Cytotoxicity, Differentiation, Maturation, Migration, Natural Killer cells, Precursors, Tissues",

author = "Monica Parodi and Raffaella Meazza and Chiara Vitale and Gabriella Pietra and Paolo Carrega and Massimo Vitale",

year = "2019",

month = jan,

day = "1",

doi = "10.1007/978-1-4939-8885-3_6",

language = "English",

volume = "1884",

pages = "87--117",

journal = "Methods in Molecular Biology",

issn = "1064-3745",

publisher = "Humana Press",

}

TY - JOUR

T1 - Isolation, Expansion, and Characterization of Natural Killer Cells and Their Precursors as a Tool to Study Cancer Immunosurveillance

AU - Parodi, Monica

AU - Meazza, Raffaella

AU - Vitale, Chiara

AU - Pietra, Gabriella

AU - Carrega, Paolo

AU - Vitale, Massimo

PY - 2019/1/1

Y1 - 2019/1/1

N2 - This chapter will describe the current methodologies to isolate and expand NK cells from Peripheral Blood (PB) or tissues for "in vitro" studies, including NK cell antitumor immune function. In addition, methods to induce NK cell maturation, differentiation, and expansion from CD34+ precursors will also be described. Finally, it will also be treated the topical issue of the characterization of new functionally and phenotypically defined NK cell subsets.

AB - This chapter will describe the current methodologies to isolate and expand NK cells from Peripheral Blood (PB) or tissues for "in vitro" studies, including NK cell antitumor immune function. In addition, methods to induce NK cell maturation, differentiation, and expansion from CD34+ precursors will also be described. Finally, it will also be treated the topical issue of the characterization of new functionally and phenotypically defined NK cell subsets.

KW - Cell isolation

KW - Chemotaxis

KW - Cytofluorimetric analysis

KW - Cytokines

KW - Cytotoxicity

KW - Differentiation

KW - Maturation

KW - Migration

KW - Natural Killer cells

KW - Precursors

KW - Tissues

UR - http://www.scopus.com/inward/record.url?scp=85056975054&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85056975054&partnerID=8YFLogxK

U2 - 10.1007/978-1-4939-8885-3_6

DO - 10.1007/978-1-4939-8885-3_6

M3 - Article

C2 - 30465197

AN - SCOPUS:85056975054

VL - 1884

SP - 87

EP - 117

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

SN - 1064-3745

ER -

Isolation, Expansion, and Characterization of Natural Killer Cells and Their Precursors as a Tool to Study Cancer Immunosurveillance

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this