Isolation, expansion, and immortalization of human adipose-derived mesenchymal stromal cells from biopsies and liposuction specimens

Luigi Balducci, Giulio Alessandri

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Human adipose tissue has proven to be an abundant, accessible, and rich source of adult mesenchymal stromal cells, suitable for tissue engineering and regenerative medicine. However, a major complication in fully investigating these cells may derive from their limited life span. Although methods to isolate, expand, and immortalize these cells have been widely reported in the literature, exhaustive explanations on the problems that can be encountered during these processes and how these can be solved have never been described. It is of fundamental importance to follow a common protocol to achieve reliable and reproducible results. Here, we describe a protocol to isolate and expand human adipose stromal cells from specimens obtained from tissue biopsies and liposuction surgical interventions. Finally, we broadly describe the cell immortalization technique, and particular attention is paid to some of the apparently “secondary” aspects.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages259-274
Number of pages16
Volume1416
DOIs
Publication statusPublished - 2016

Publication series

NameMethods in Molecular Biology
Volume1416
ISSN (Print)10643745

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Keywords

  • Adipose-derived stromal cells
  • Enzymatic digestion
  • Expansion
  • Fat biopsy
  • Human adipose tissue
  • Immortalization
  • Isolation
  • Liposuction
  • Stromal vascular fraction

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Balducci, L., & Alessandri, G. (2016). Isolation, expansion, and immortalization of human adipose-derived mesenchymal stromal cells from biopsies and liposuction specimens. In Methods in Molecular Biology (Vol. 1416, pp. 259-274). (Methods in Molecular Biology; Vol. 1416). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-3584-0_15