Isolation, expansion, and immortalization of human adipose-derived mesenchymal stromal cells from biopsies and liposuction specimens

Luigi Balducci, Giulio Alessandri

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Citations (Scopus)

Abstract

Human adipose tissue has proven to be an abundant, accessible, and rich source of adult mesenchymal stromal cells, suitable for tissue engineering and regenerative medicine. However, a major complication in fully investigating these cells may derive from their limited life span. Although methods to isolate, expand, and immortalize these cells have been widely reported in the literature, exhaustive explanations on the problems that can be encountered during these processes and how these can be solved have never been described. It is of fundamental importance to follow a common protocol to achieve reliable and reproducible results. Here, we describe a protocol to isolate and expand human adipose stromal cells from specimens obtained from tissue biopsies and liposuction surgical interventions. Finally, we broadly describe the cell immortalization technique, and particular attention is paid to some of the apparently “secondary” aspects.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages259-274
Number of pages16
Volume1416
DOIs
Publication statusPublished - 2016

Publication series

NameMethods in Molecular Biology
Volume1416
ISSN (Print)10643745

Fingerprint

Lipectomy
Mesenchymal Stromal Cells
Biopsy
Regenerative Medicine
Tissue Engineering
Stromal Cells
Adipose Tissue

Keywords

  • Adipose-derived stromal cells
  • Enzymatic digestion
  • Expansion
  • Fat biopsy
  • Human adipose tissue
  • Immortalization
  • Isolation
  • Liposuction
  • Stromal vascular fraction

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Balducci, L., & Alessandri, G. (2016). Isolation, expansion, and immortalization of human adipose-derived mesenchymal stromal cells from biopsies and liposuction specimens. In Methods in Molecular Biology (Vol. 1416, pp. 259-274). (Methods in Molecular Biology; Vol. 1416). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-3584-0_15

Isolation, expansion, and immortalization of human adipose-derived mesenchymal stromal cells from biopsies and liposuction specimens. / Balducci, Luigi; Alessandri, Giulio.

Methods in Molecular Biology. Vol. 1416 Humana Press Inc., 2016. p. 259-274 (Methods in Molecular Biology; Vol. 1416).

Research output: Chapter in Book/Report/Conference proceedingChapter

Balducci, L & Alessandri, G 2016, Isolation, expansion, and immortalization of human adipose-derived mesenchymal stromal cells from biopsies and liposuction specimens. in Methods in Molecular Biology. vol. 1416, Methods in Molecular Biology, vol. 1416, Humana Press Inc., pp. 259-274. https://doi.org/10.1007/978-1-4939-3584-0_15
Balducci L, Alessandri G. Isolation, expansion, and immortalization of human adipose-derived mesenchymal stromal cells from biopsies and liposuction specimens. In Methods in Molecular Biology. Vol. 1416. Humana Press Inc. 2016. p. 259-274. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-3584-0_15
Balducci, Luigi ; Alessandri, Giulio. / Isolation, expansion, and immortalization of human adipose-derived mesenchymal stromal cells from biopsies and liposuction specimens. Methods in Molecular Biology. Vol. 1416 Humana Press Inc., 2016. pp. 259-274 (Methods in Molecular Biology).
@inbook{8b1c3231a84640a8b0373665ff2ca4f2,
title = "Isolation, expansion, and immortalization of human adipose-derived mesenchymal stromal cells from biopsies and liposuction specimens",
abstract = "Human adipose tissue has proven to be an abundant, accessible, and rich source of adult mesenchymal stromal cells, suitable for tissue engineering and regenerative medicine. However, a major complication in fully investigating these cells may derive from their limited life span. Although methods to isolate, expand, and immortalize these cells have been widely reported in the literature, exhaustive explanations on the problems that can be encountered during these processes and how these can be solved have never been described. It is of fundamental importance to follow a common protocol to achieve reliable and reproducible results. Here, we describe a protocol to isolate and expand human adipose stromal cells from specimens obtained from tissue biopsies and liposuction surgical interventions. Finally, we broadly describe the cell immortalization technique, and particular attention is paid to some of the apparently “secondary” aspects.",
keywords = "Adipose-derived stromal cells, Enzymatic digestion, Expansion, Fat biopsy, Human adipose tissue, Immortalization, Isolation, Liposuction, Stromal vascular fraction",
author = "Luigi Balducci and Giulio Alessandri",
year = "2016",
doi = "10.1007/978-1-4939-3584-0_15",
language = "English",
volume = "1416",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "259--274",
booktitle = "Methods in Molecular Biology",

}

TY - CHAP

T1 - Isolation, expansion, and immortalization of human adipose-derived mesenchymal stromal cells from biopsies and liposuction specimens

AU - Balducci, Luigi

AU - Alessandri, Giulio

PY - 2016

Y1 - 2016

N2 - Human adipose tissue has proven to be an abundant, accessible, and rich source of adult mesenchymal stromal cells, suitable for tissue engineering and regenerative medicine. However, a major complication in fully investigating these cells may derive from their limited life span. Although methods to isolate, expand, and immortalize these cells have been widely reported in the literature, exhaustive explanations on the problems that can be encountered during these processes and how these can be solved have never been described. It is of fundamental importance to follow a common protocol to achieve reliable and reproducible results. Here, we describe a protocol to isolate and expand human adipose stromal cells from specimens obtained from tissue biopsies and liposuction surgical interventions. Finally, we broadly describe the cell immortalization technique, and particular attention is paid to some of the apparently “secondary” aspects.

AB - Human adipose tissue has proven to be an abundant, accessible, and rich source of adult mesenchymal stromal cells, suitable for tissue engineering and regenerative medicine. However, a major complication in fully investigating these cells may derive from their limited life span. Although methods to isolate, expand, and immortalize these cells have been widely reported in the literature, exhaustive explanations on the problems that can be encountered during these processes and how these can be solved have never been described. It is of fundamental importance to follow a common protocol to achieve reliable and reproducible results. Here, we describe a protocol to isolate and expand human adipose stromal cells from specimens obtained from tissue biopsies and liposuction surgical interventions. Finally, we broadly describe the cell immortalization technique, and particular attention is paid to some of the apparently “secondary” aspects.

KW - Adipose-derived stromal cells

KW - Enzymatic digestion

KW - Expansion

KW - Fat biopsy

KW - Human adipose tissue

KW - Immortalization

KW - Isolation

KW - Liposuction

KW - Stromal vascular fraction

UR - http://www.scopus.com/inward/record.url?scp=84990220488&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84990220488&partnerID=8YFLogxK

U2 - 10.1007/978-1-4939-3584-0_15

DO - 10.1007/978-1-4939-3584-0_15

M3 - Chapter

VL - 1416

T3 - Methods in Molecular Biology

SP - 259

EP - 274

BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -