Isolation from the horse genome of a new DNA transposon belonging to the Tigger family

Marianna Paulis, Daniela Moralli, Mirella Bensi, Luigi De Carli, Elena Raimondi

Research output: Contribution to journalArticlepeer-review

Abstract

Tigger elements are human DNA transposons homologous to the pogo element of Drosophila melanogaster. They contain an open reading frame for a transposase very similar to the major mammalian centromere protein CENP-B. We found in the horse genome a DNA element (Ecatig3) sharing 88% homology with human Tigger3. The presence of Tigger elements in the horse genome confirms previous data that date these elements before the divergence between Perissodactyla and Primates (80-90 Myr ago). Copy number evaluation indicates that the horse element is much more abundant than its human counterpart. Southern blot analysis demonstrates that Ecatig3 elements are extremely homogeneous; this may indicate that the evolution of this DNA transposon has been driven by some kind of selection and has not been neutral.

Original languageEnglish
Pages (from-to)399-403
Number of pages5
JournalMammalian Genome
Volume15
Issue number5
DOIs
Publication statusPublished - May 2004

ASJC Scopus subject areas

  • Genetics

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