Isolation of a pancreas-specific gene located on human chromosome 14q31: Expression analysis in human pancreatic ductal carcinomas

Ida Biunno, Valentina Appierto, Monica Cattaneo, Biagio Eugenic Leone, GianPaolo Balzano, Carlo Socci, Salvatore Saccone, Andreozzi Letizia, Giuliano Della Valle, Vittorio Sgaramella

Research output: Contribution to journalArticlepeer-review

Abstract

We have isolated a novel cDNA (SEL1L) that shows sequence similarities to SEL-1, a gene identified as an extragenic suppressor of the lin-12 hypomorphic mutant from Caenorhabditis elegans (7, 8). SEL1L exhibits a tissue-specific pattern of expression: a single poly(A)+ RNA species of 7.5 kb is abundantly expressed only in the pancreas of healthy individuals, whereas low to undetectable levels are observed in other adult and in some fetal tissues. Somatic hybrid panel and fluorescence in situ hybridization positioned this gene in the q31 hand of human chromosome 14. The tissue- specific expression of this gene induced us to study its role in human pancreatic carcinomas. Our analysis revealed that 17% of adenocarcinomas of the pancreas did not express SEL1L to a detectable level; however, no gross genomic alterations were apparent in the few hundred kilobases of the relevant region.

Original languageEnglish
Pages (from-to)284-286
Number of pages3
JournalGenomics
Volume46
Issue number2
DOIs
Publication statusPublished - Dec 1 1997

Keywords

  • Human chromosome 14
  • Pancreatic ductal carcinoma
  • Tissue-specific gene

ASJC Scopus subject areas

  • Genetics

Fingerprint Dive into the research topics of 'Isolation of a pancreas-specific gene located on human chromosome 14q31: Expression analysis in human pancreatic ductal carcinomas'. Together they form a unique fingerprint.

Cite this