TY - JOUR
T1 - Isolation of a pancreas-specific gene located on human chromosome 14q31
T2 - Expression analysis in human pancreatic ductal carcinomas
AU - Biunno, Ida
AU - Appierto, Valentina
AU - Cattaneo, Monica
AU - Leone, Biagio Eugenic
AU - Balzano, GianPaolo
AU - Socci, Carlo
AU - Saccone, Salvatore
AU - Letizia, Andreozzi
AU - Della Valle, Giuliano
AU - Sgaramella, Vittorio
PY - 1997/12/1
Y1 - 1997/12/1
N2 - We have isolated a novel cDNA (SEL1L) that shows sequence similarities to SEL-1, a gene identified as an extragenic suppressor of the lin-12 hypomorphic mutant from Caenorhabditis elegans (7, 8). SEL1L exhibits a tissue-specific pattern of expression: a single poly(A)+ RNA species of 7.5 kb is abundantly expressed only in the pancreas of healthy individuals, whereas low to undetectable levels are observed in other adult and in some fetal tissues. Somatic hybrid panel and fluorescence in situ hybridization positioned this gene in the q31 hand of human chromosome 14. The tissue- specific expression of this gene induced us to study its role in human pancreatic carcinomas. Our analysis revealed that 17% of adenocarcinomas of the pancreas did not express SEL1L to a detectable level; however, no gross genomic alterations were apparent in the few hundred kilobases of the relevant region.
AB - We have isolated a novel cDNA (SEL1L) that shows sequence similarities to SEL-1, a gene identified as an extragenic suppressor of the lin-12 hypomorphic mutant from Caenorhabditis elegans (7, 8). SEL1L exhibits a tissue-specific pattern of expression: a single poly(A)+ RNA species of 7.5 kb is abundantly expressed only in the pancreas of healthy individuals, whereas low to undetectable levels are observed in other adult and in some fetal tissues. Somatic hybrid panel and fluorescence in situ hybridization positioned this gene in the q31 hand of human chromosome 14. The tissue- specific expression of this gene induced us to study its role in human pancreatic carcinomas. Our analysis revealed that 17% of adenocarcinomas of the pancreas did not express SEL1L to a detectable level; however, no gross genomic alterations were apparent in the few hundred kilobases of the relevant region.
KW - Human chromosome 14
KW - Pancreatic ductal carcinoma
KW - Tissue-specific gene
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U2 - 10.1006/geno.1997.5018
DO - 10.1006/geno.1997.5018
M3 - Article
C2 - 9417916
AN - SCOPUS:0031466399
VL - 46
SP - 284
EP - 286
JO - Genomics
JF - Genomics
SN - 0888-7543
IS - 2
ER -