Isolation of a SIR-like gene, SIR-T8, that is overexpressed in thyroid carcinoma cell lines and tissues

F. De Nigris, J. Cerutti, C. Morelli, D. Califano, L. Chiariotti, G. Viglietto, G. Santelli, A. Fusco

Research output: Contribution to journalArticlepeer-review

Abstract

We used subtractive library screening to identify the changes that occur in gene expression during thyroid cell neoplastic transformation. Complementary DNA from normal thyroid cells (HTC 2) was subtracted from a complementary DNA library constructed from a human thyroid papillary carcinoma cell line. The library was screened for genes upregulated in human thyroid papillary carcinoma cell line cells, and several cDNA clones were isolated. One of these clones has a sirtuin core and high homology with the human silent information regulator protein family. This clone, designated 'SIR-TS', was overexpressed in human thyroid carcinoma cell lines and tissues, but not in adenomas. The human SIR-T8 protein has a molecular weight of 39 kDa and is primarily located in the cytoplasm under the nuclear membrane. The SIR-T8 gene is located on chromosome 17q25-1.

Original languageEnglish
Pages (from-to)917-923
Number of pages7
JournalBritish Journal of Cancer
Volume86
Issue number6
DOIs
Publication statusPublished - Mar 18 2002

Keywords

  • Expression
  • Neoplasias
  • SIR2
  • Telomerase
  • Thyroid

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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