Isolation of an avianlike group A rotavirus from a calf with diarrhea

H. Brussow, O. Nakagomi, G. Gerna, W. Eichhorn

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Abstract

An atypical group A rotavirus (993/83) was isolated from a 3-day-old German calf with diarrhea. It differed from 35 conventional German bovine rotavirus isolates analyzed previously with respect to subgroup (strain 993/83 was non-subgroup I and non-subgroup II), serotype (strain 993/83 showed a two-way cross-reaction with serotype 7 and a one-way cross-reaction with serotype 3), and electropherotype (strain 993/83 showed comigrating gene segments 10 and 11). Isolate 993/83 reacted with only one of four monoclonal antibodies that recognized a common VP6 epitope(s). In addition, VP6 and VP2 of isolate 993/83 showed one-dimensional peptide maps that differed substantially from the peptide maps of VP6 and VP2 from all bovine rotavirus isolates. By RNA-RNA hybridization, the 993/83 probe failed to react with a panel of mammalian rotavirus strains, including bovine rotaviruses. It hybridized, however, to genomic RNA of an avian rotavirus strain. Isolate 993/83 could thus represent a candidate for a natural interspecies transmission of rotavirus between different classes of vertebrates.

Original languageEnglish
Pages (from-to)67-73
Number of pages7
JournalJournal of Clinical Microbiology
Volume30
Issue number1
Publication statusPublished - 1992

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Rotavirus
Diarrhea
Cross Reactions
RNA
Peptides
Vertebrates
Epitopes
Monoclonal Antibodies
Genes
Serogroup

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Isolation of an avianlike group A rotavirus from a calf with diarrhea. / Brussow, H.; Nakagomi, O.; Gerna, G.; Eichhorn, W.

In: Journal of Clinical Microbiology, Vol. 30, No. 1, 1992, p. 67-73.

Research output: Contribution to journalArticle

Brussow, H, Nakagomi, O, Gerna, G & Eichhorn, W 1992, 'Isolation of an avianlike group A rotavirus from a calf with diarrhea', Journal of Clinical Microbiology, vol. 30, no. 1, pp. 67-73.
Brussow, H. ; Nakagomi, O. ; Gerna, G. ; Eichhorn, W. / Isolation of an avianlike group A rotavirus from a calf with diarrhea. In: Journal of Clinical Microbiology. 1992 ; Vol. 30, No. 1. pp. 67-73.
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