Isolation of cDNA clones corresponding to the Mr = 150,000 subunit of chick type VI collagen

Paolo Bonaldo, Francesco Bucciotti, Alfonso Colombatti

Research output: Contribution to journalArticle

Abstract

Type VI collagen is a disulfide-bonded protein with an unusual structure in that the molecule contains three short triplehelical domains and very extended non-collagenous regions. The molecule is a heterotrimer composed in the chick of two polypeptides of similar apparent size in SDS-PAGE (Mr = 140- and 150,000) but different structure, and a third component that is much larger (Mr = 260,000) than the other two chains. We report here on the isolation of several overlapping cDNA clones from a chicken aorta mRNA expression library in the plasmid vector pEX1. Antibodies affinity purified onto the fusion proteins recognized the chick type VI collagen Mr = 150,000 subunit. Northern blots using the cDNA inserts from the above clones revealed a single RNA species of about 4,600 nucleotides sufficient to code for a protein with the size of the Mr = 150,000 subunit.

Original languageEnglish
Pages (from-to)347-354
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume149
Issue number2
DOIs
Publication statusPublished - Dec 16 1987

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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