Isolation of cDNA clones corresponding to the Mr = 150,000 subunit of chick type VI collagen

Paolo Bonaldo, Francesco Bucciotti, Alfonso Colombatti

Research output: Contribution to journalArticle

Abstract

Type VI collagen is a disulfide-bonded protein with an unusual structure in that the molecule contains three short triplehelical domains and very extended non-collagenous regions. The molecule is a heterotrimer composed in the chick of two polypeptides of similar apparent size in SDS-PAGE (Mr = 140- and 150,000) but different structure, and a third component that is much larger (Mr = 260,000) than the other two chains. We report here on the isolation of several overlapping cDNA clones from a chicken aorta mRNA expression library in the plasmid vector pEX1. Antibodies affinity purified onto the fusion proteins recognized the chick type VI collagen Mr = 150,000 subunit. Northern blots using the cDNA inserts from the above clones revealed a single RNA species of about 4,600 nucleotides sufficient to code for a protein with the size of the Mr = 150,000 subunit.

Original languageEnglish
Pages (from-to)347-354
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume149
Issue number2
DOIs
Publication statusPublished - Dec 16 1987

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Collagen Type VI
Complementary DNA
Clone Cells
Proteins
Molecules
Antibody Affinity
Disulfides
Northern Blotting
Aorta
Polyacrylamide Gel Electrophoresis
Chickens
Plasmids
Fusion reactions
Nucleotides
RNA
Messenger RNA
Peptides
Antibodies

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Isolation of cDNA clones corresponding to the Mr = 150,000 subunit of chick type VI collagen. / Bonaldo, Paolo; Bucciotti, Francesco; Colombatti, Alfonso.

In: Biochemical and Biophysical Research Communications, Vol. 149, No. 2, 16.12.1987, p. 347-354.

Research output: Contribution to journalArticle

Bonaldo, Paolo ; Bucciotti, Francesco ; Colombatti, Alfonso. / Isolation of cDNA clones corresponding to the Mr = 150,000 subunit of chick type VI collagen. In: Biochemical and Biophysical Research Communications. 1987 ; Vol. 149, No. 2. pp. 347-354.
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