Isolation of chromosome-specific genes by reciprocal probing of arrayed cDNA and cosmid libraries

Cheng Chi Lee, Ali Yazdani, Manfred Wehnert, Zhaoyang Zhao, Elizabeth A. Lindsay, Jennifer Bailey, Mary I. Coolbaugh, Lisa Couch, Mengli Xiong, A. Craig Chinault, Antonio Baldini, C. Thomas Caskey

Research output: Contribution to journalArticle

Abstract

We have identified and mapped 61 novel and previously described chromosome 17 and X genes, using a human placental cDNA library. These genes were isolated using a gene identification and mapping strategy based on reciprocal probing of arrayed chromosome specific cosmid and cDNA libraries. This strategy scans gridded cosmids for nuclear genes and isolates the expressed sequence by a cosmid to cDNA filter hybridization. Inherent to this approach is the advantage of identifying the corresponding genomic cosmid clone of a particular cDNA. The genomic and cDNA reagents can be used for fluorescence in situ hybridization (FISH) and polymerase chain reaction (PCR) based mapping to resolve map positions of cDNAs belonging to gene families and those associated with multiple chromosomes. The downstream utility of reagents generated by the reciprocal probing methods is demonstrated in our studies.

Original languageEnglish
Pages (from-to)1373-1380
Number of pages8
JournalHuman Molecular Genetics
Volume4
Issue number8
DOIs
Publication statusPublished - Aug 1995

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Public Health, Environmental and Occupational Health
  • Molecular Biology
  • Genetics(clinical)
  • Genetics

Fingerprint Dive into the research topics of 'Isolation of chromosome-specific genes by reciprocal probing of arrayed cDNA and cosmid libraries'. Together they form a unique fingerprint.

  • Cite this

    Lee, C. C., Yazdani, A., Wehnert, M., Zhao, Z., Lindsay, E. A., Bailey, J., Coolbaugh, M. I., Couch, L., Xiong, M., Chinault, A. C., Baldini, A., & Caskey, C. T. (1995). Isolation of chromosome-specific genes by reciprocal probing of arrayed cDNA and cosmid libraries. Human Molecular Genetics, 4(8), 1373-1380. https://doi.org/10.1093/hmg/4.8.1373