Isolation of chromosome-specific genes by reciprocal probing of arrayed cDNA and cosmid libraries

Cheng Chi Lee, Ali Yazdani, Manfred Wehnert, Zhaoyang Zhao, Elizabeth A. Lindsay, Jennifer Bailey, Mary I. Coolbaugh, Lisa Couch, Mengli Xiong, A. Craig Chinault, Antonio Baldini, C. Thomas Caskey

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

We have identified and mapped 61 novel and previously described chromosome 17 and X genes, using a human placental cDNA library. These genes were isolated using a gene identification and mapping strategy based on reciprocal probing of arrayed chromosome specific cosmid and cDNA libraries. This strategy scans gridded cosmids for nuclear genes and isolates the expressed sequence by a cosmid to cDNA filter hybridization. Inherent to this approach is the advantage of identifying the corresponding genomic cosmid clone of a particular cDNA. The genomic and cDNA reagents can be used for fluorescence in situ hybridization (FISH) and polymerase chain reaction (PCR) based mapping to resolve map positions of cDNAs belonging to gene families and those associated with multiple chromosomes. The downstream utility of reagents generated by the reciprocal probing methods is demonstrated in our studies.

Original languageEnglish
Pages (from-to)1373-1380
Number of pages8
JournalHuman Molecular Genetics
Volume4
Issue number8
DOIs
Publication statusPublished - Aug 1995

Fingerprint

Cosmids
Chromosomes
CDNA
Gene Library
Chromosome
Isolation
Complementary DNA
Genes
Gene
Chromosomes, Human, Pair 17
Genomics
Chromosome Mapping
Fluorescence In Situ Hybridization
Polymerase chain reaction
In Situ Hybridization
Polymerase Chain Reaction
Clone Cells
Clone
Fluorescence
Libraries

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Public Health, Environmental and Occupational Health
  • Molecular Biology
  • Genetics(clinical)
  • Genetics

Cite this

Lee, C. C., Yazdani, A., Wehnert, M., Zhao, Z., Lindsay, E. A., Bailey, J., ... Caskey, C. T. (1995). Isolation of chromosome-specific genes by reciprocal probing of arrayed cDNA and cosmid libraries. Human Molecular Genetics, 4(8), 1373-1380. https://doi.org/10.1093/hmg/4.8.1373

Isolation of chromosome-specific genes by reciprocal probing of arrayed cDNA and cosmid libraries. / Lee, Cheng Chi; Yazdani, Ali; Wehnert, Manfred; Zhao, Zhaoyang; Lindsay, Elizabeth A.; Bailey, Jennifer; Coolbaugh, Mary I.; Couch, Lisa; Xiong, Mengli; Chinault, A. Craig; Baldini, Antonio; Caskey, C. Thomas.

In: Human Molecular Genetics, Vol. 4, No. 8, 08.1995, p. 1373-1380.

Research output: Contribution to journalArticle

Lee, CC, Yazdani, A, Wehnert, M, Zhao, Z, Lindsay, EA, Bailey, J, Coolbaugh, MI, Couch, L, Xiong, M, Chinault, AC, Baldini, A & Caskey, CT 1995, 'Isolation of chromosome-specific genes by reciprocal probing of arrayed cDNA and cosmid libraries', Human Molecular Genetics, vol. 4, no. 8, pp. 1373-1380. https://doi.org/10.1093/hmg/4.8.1373
Lee, Cheng Chi ; Yazdani, Ali ; Wehnert, Manfred ; Zhao, Zhaoyang ; Lindsay, Elizabeth A. ; Bailey, Jennifer ; Coolbaugh, Mary I. ; Couch, Lisa ; Xiong, Mengli ; Chinault, A. Craig ; Baldini, Antonio ; Caskey, C. Thomas. / Isolation of chromosome-specific genes by reciprocal probing of arrayed cDNA and cosmid libraries. In: Human Molecular Genetics. 1995 ; Vol. 4, No. 8. pp. 1373-1380.
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