Isolation of Crb1, a mouse homologue of Drosophila crumbs, and analysis of its expression pattern in eye and brain

Anneke I. Den Hollander, Michela Ghiani, Yvette J M De Kok, Jan Wijnholds, Andrea Ballabio, Frans P M Cremers, Vania Broccoli

Research output: Contribution to journalArticle

Abstract

Mutations in the human Crumbs homologue 1 (CRB1) gene cause severe retinal dystrophies. CRB1 is homologous to Drosophila Crumbs, a protein essential for establishing and maintaining epithelial polarity. We have isolated the mouse orthologue, Crb1, and analyzed its expression pattern in embryonic and post-natal stages. Crb1 is expressed exclusively in the eye, and the central nervous system. In the developing eye, expression of Crb1 is detected in the retinal progenitors, and later on becomes restricted to the differentiated photoreceptor cells where it remains active up to the adult stage. In the developing neural tube, expression of Crb1 is restricted to its most ventral structures, coinciding with the expression domain of Nkx2.2. In the adult brain, Crb1 expression is defined to areas where the production and migration of neurons occurs in adulthood.

Original languageEnglish
Pages (from-to)203-207
Number of pages5
JournalMechanisms of Development
Volume110
Issue number1-2
DOIs
Publication statusPublished - 2001

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Keywords

  • Apico-basal polarity
  • Bipolar neurons
  • Brain
  • Central nervous system
  • Crumbs
  • Crumbs homologue 1
  • Dentate gyrus
  • Epithelial polarity
  • Eye
  • Hypothalamus mammillary bodies
  • Inner nuclear layer
  • Iris
  • Leber congenital amaurosis
  • Neural stem cell
  • Neural tube
  • Nkx2.2
  • Olfactory bulbs
  • Outer nuclear layer
  • Photoreceptor
  • Retina
  • Retinal progenitor
  • Retinitis pigmentosa
  • Retinoblasts
  • Rostral migratory stream
  • Sonic hedge hog
  • Subventricular region
  • V3 interneurons
  • Zona limitans intrathalamica

ASJC Scopus subject areas

  • Developmental Biology
  • Developmental Neuroscience

Cite this

Den Hollander, A. I., Ghiani, M., De Kok, Y. J. M., Wijnholds, J., Ballabio, A., Cremers, F. P. M., & Broccoli, V. (2001). Isolation of Crb1, a mouse homologue of Drosophila crumbs, and analysis of its expression pattern in eye and brain. Mechanisms of Development, 110(1-2), 203-207. https://doi.org/10.1016/S0925-4773(01)00568-8