Isolation of intermediate valence hybrids between ferrous and methemoglobin at subzero temperatures.

M. Perrella, L. Cremonesi, L. Benazzi, L. Rossi-Bernardi

Research output: Contribution to journalArticlepeer-review

Abstract

Quenching a hemoglobin solution partially saturated with carbon monoxide into a hydro-organic solvent containing ferricyanide will produce under suitable conditions a population of partially oxidized and CO-bound hemoglobin molecules. Since each Fe3+ heme carries one extra charge, it should be possible, in theory, to resolve the spectrum of intermediate compounds between hemoglobin and carbon monoxide, which was originally present in solution. In this study we report: 1) the development of a simple and rapid method to quench aqueous hemoglobin solutions into a hydro-organic solvent at subzero temperatures; 2) the determination of suitable experimental conditions to isolate valence hybrids between carbonmonoxy- and methemoglobin by isoelectric focusing at temperatures as low as -25 degrees C; and 3) the identification and isolation of all valence hybrids of different charge between carbonmonoxy- and methemoglobin.

Original languageEnglish
Pages (from-to)11098-11103
Number of pages6
JournalJournal of Biological Chemistry
Volume256
Issue number21
Publication statusPublished - Nov 10 1981

ASJC Scopus subject areas

  • Biochemistry

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