Isolation of mitochondria for biogenetical studies: An update

Erika Fernández-Vizarra; Gustavo Ferrín; Acisclo Pérez-Martos; Patricio Fernández-Silva; Massimo Zeviani; José Antonio Enríquez

doi:10.1016/j.mito.2009.12.148

Isolation of mitochondria for biogenetical studies: An update

Erika Fernández-Vizarra, Gustavo Ferrín, Acisclo Pérez-Martos, Patricio Fernández-Silva, Massimo Zeviani, José Antonio Enríquez

Fondazione IRCCS Istituto Neurologico "C. Besta"

Research output: Contribution to journal › Article › peer-review

Abstract

The use of good quality preparations of isolated mitochondria is necessary when studying the mitochondrial biogenetical activities. This article explains a fast and simple method for the purification of mammalian mitochondria from different tissues and cultured cells, that is suitable for the analysis of many aspects of the organelle's biogenesis. The mitochondria isolated following the protocol described here, are highly active and capable of DNA, RNA and protein synthesis. Mitochondrial tRNA aminoacylation, mtDNA-protein interactions and specific import of added proteins into the organelles, can also be studied using this kind of preparations.

Original language	English
Pages (from-to)	253-262
Number of pages	10
Journal	Mitochondrion
Volume	10
Issue number	3
DOIs	https://doi.org/10.1016/j.mito.2009.12.148
Publication status	Published - Apr 2010

Keywords

In organello assays
Isolation of mitochondria
MtDNA expression studies
Protein import

ASJC Scopus subject areas

Cell Biology
Molecular Biology
Molecular Medicine

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AU - Ferrín, Gustavo

AU - Pérez-Martos, Acisclo

AU - Fernández-Silva, Patricio

AU - Zeviani, Massimo

AU - Enríquez, José Antonio

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KW - MtDNA expression studies

KW - Protein import

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