Abstract
The use of good quality preparations of isolated mitochondria is necessary when studying the mitochondrial biogenetical activities. This article explains a fast and simple method for the purification of mammalian mitochondria from different tissues and cultured cells, that is suitable for the analysis of many aspects of the organelle's biogenesis. The mitochondria isolated following the protocol described here, are highly active and capable of DNA, RNA and protein synthesis. Mitochondrial tRNA aminoacylation, mtDNA-protein interactions and specific import of added proteins into the organelles, can also be studied using this kind of preparations.
| Original language | English |
|---|---|
| Pages (from-to) | 253-262 |
| Number of pages | 10 |
| Journal | Mitochondrion |
| Volume | 10 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Apr 2010 |
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Keywords
- In organello assays
- Isolation of mitochondria
- MtDNA expression studies
- Protein import
ASJC Scopus subject areas
- Cell Biology
- Molecular Biology
- Molecular Medicine
Cite this
Isolation of mitochondria for biogenetical studies : An update. / Fernández-Vizarra, Erika; Ferrín, Gustavo; Pérez-Martos, Acisclo; Fernández-Silva, Patricio; Zeviani, Massimo; Enríquez, José Antonio.
In: Mitochondrion, Vol. 10, No. 3, 04.2010, p. 253-262.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Isolation of mitochondria for biogenetical studies
T2 - An update
AU - Fernández-Vizarra, Erika
AU - Ferrín, Gustavo
AU - Pérez-Martos, Acisclo
AU - Fernández-Silva, Patricio
AU - Zeviani, Massimo
AU - Enríquez, José Antonio
PY - 2010/4
Y1 - 2010/4
N2 - The use of good quality preparations of isolated mitochondria is necessary when studying the mitochondrial biogenetical activities. This article explains a fast and simple method for the purification of mammalian mitochondria from different tissues and cultured cells, that is suitable for the analysis of many aspects of the organelle's biogenesis. The mitochondria isolated following the protocol described here, are highly active and capable of DNA, RNA and protein synthesis. Mitochondrial tRNA aminoacylation, mtDNA-protein interactions and specific import of added proteins into the organelles, can also be studied using this kind of preparations.
AB - The use of good quality preparations of isolated mitochondria is necessary when studying the mitochondrial biogenetical activities. This article explains a fast and simple method for the purification of mammalian mitochondria from different tissues and cultured cells, that is suitable for the analysis of many aspects of the organelle's biogenesis. The mitochondria isolated following the protocol described here, are highly active and capable of DNA, RNA and protein synthesis. Mitochondrial tRNA aminoacylation, mtDNA-protein interactions and specific import of added proteins into the organelles, can also be studied using this kind of preparations.
KW - In organello assays
KW - Isolation of mitochondria
KW - MtDNA expression studies
KW - Protein import
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UR - http://www.scopus.com/inward/citedby.url?scp=77949916467&partnerID=8YFLogxK
U2 - 10.1016/j.mito.2009.12.148
DO - 10.1016/j.mito.2009.12.148
M3 - Article
C2 - 20034597
AN - SCOPUS:77949916467
VL - 10
SP - 253
EP - 262
JO - Mitochondrion
JF - Mitochondrion
SN - 1567-7249
IS - 3
ER -