Isolation of MUC1-primed B lymphocytes from tumour-draining lymph nodes by immunomagnetic beads

Claudia Petrarca, Beniamino Casalino, Silvia Von Mensdorff-Pouilly, Aurelia Rughetti, Hassan Rahimi, Giovanni Scambia, Joseph Hilgers, Luigi Frati, Marianna Nuti

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

The humoral immune response against a tumour-associated antigen, polymorphic epithelial mucin (PEM, MUC1) in cancer patients was studied by isolating specific B cells primed for the antigen. Human B lymphocytes from tumour-draining lymph nodes, obtained from 12 patients with epithelial cancers, were immunoselected with magnetic beads coated with a 60mer synthetic peptide corresponding to three tandem repeats of the protein core of the MUC1 antigen. Short-term cultures of B cells were established utilizing interleukin-10 (IL-10), IL-4 and monoclonal antibody anti-CD40, and were maintained for a maximum of 3 weeks. B cell culture supernatants contained human anti-MUC1 antibodies, as detected by enzyme-linked immunosorbent assay, in 6/12 of the patients tested. Five of these patients, all with early-stage cancer, also had high levels of circulating anti-MUC1 IgM antibodies in the serum. A significant correlation was found (two-tailed P = 0.041) between the presence of circulating anti-MUC1 antibodies and the ability to isolate PEM-specific B cells from tumour-draining lymph nodes. The technique proposed provides a useful method for the analysis of natural immunity against defined tumour antigens.

Original languageEnglish
Pages (from-to)272-277
Number of pages6
JournalCancer Immunology, Immunotherapy
Volume47
Issue number5
DOIs
Publication statusPublished - 1999

Fingerprint

B-Lymphocytes
Lymph Nodes
Neoplasm Antigens
Neoplasms
Anti-Idiotypic Antibodies
Antigens
Mucin-1
Tandem Repeat Sequences
Humoral Immunity
Innate Immunity
Interleukin-4
Interleukin-10
Cell Culture Techniques
Enzyme-Linked Immunosorbent Assay
Monoclonal Antibodies
Peptides
Antibodies
Serum
Proteins

Keywords

  • B lymphocytes
  • Immune response
  • Lymph nodes
  • MUC1
  • Polymorphic epithelial mucin
  • Tumour-associated antigen

ASJC Scopus subject areas

  • Cancer Research
  • Immunology
  • Oncology

Cite this

Petrarca, C., Casalino, B., Von Mensdorff-Pouilly, S., Rughetti, A., Rahimi, H., Scambia, G., ... Nuti, M. (1999). Isolation of MUC1-primed B lymphocytes from tumour-draining lymph nodes by immunomagnetic beads. Cancer Immunology, Immunotherapy, 47(5), 272-277. https://doi.org/10.1007/s002620050531

Isolation of MUC1-primed B lymphocytes from tumour-draining lymph nodes by immunomagnetic beads. / Petrarca, Claudia; Casalino, Beniamino; Von Mensdorff-Pouilly, Silvia; Rughetti, Aurelia; Rahimi, Hassan; Scambia, Giovanni; Hilgers, Joseph; Frati, Luigi; Nuti, Marianna.

In: Cancer Immunology, Immunotherapy, Vol. 47, No. 5, 1999, p. 272-277.

Research output: Contribution to journalArticle

Petrarca, C, Casalino, B, Von Mensdorff-Pouilly, S, Rughetti, A, Rahimi, H, Scambia, G, Hilgers, J, Frati, L & Nuti, M 1999, 'Isolation of MUC1-primed B lymphocytes from tumour-draining lymph nodes by immunomagnetic beads', Cancer Immunology, Immunotherapy, vol. 47, no. 5, pp. 272-277. https://doi.org/10.1007/s002620050531
Petrarca C, Casalino B, Von Mensdorff-Pouilly S, Rughetti A, Rahimi H, Scambia G et al. Isolation of MUC1-primed B lymphocytes from tumour-draining lymph nodes by immunomagnetic beads. Cancer Immunology, Immunotherapy. 1999;47(5):272-277. https://doi.org/10.1007/s002620050531
Petrarca, Claudia ; Casalino, Beniamino ; Von Mensdorff-Pouilly, Silvia ; Rughetti, Aurelia ; Rahimi, Hassan ; Scambia, Giovanni ; Hilgers, Joseph ; Frati, Luigi ; Nuti, Marianna. / Isolation of MUC1-primed B lymphocytes from tumour-draining lymph nodes by immunomagnetic beads. In: Cancer Immunology, Immunotherapy. 1999 ; Vol. 47, No. 5. pp. 272-277.
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