Isolation of scFv fragments to polymorphic and monomorphic HLA-DR epitopes from a synthetic phage library

Maria Pia Pistillo, Juergen Hammer, Elisa Bono, Francesco Sinigaglia, Andrea Gho, Chiara Marchisone, Giovanni Battista Ferrara

Research output: Contribution to journalArticle

Abstract

The possibility of producing human recombinant antibodies by the phage display libraries technology has opened up new perspectives in the fields of immunological research and therapeutic applications. Despite the interest for a potential use of this technology in the HLA field, no information is so far available about selection and screening of libraries with HLA antigens. In this study we report our first experience with expression and characterization of human single-chain antibody fragments (scFvs) against HLA-DR epitopes selected from a synthetic phage library.

Original languageEnglish
Pages (from-to)123-130
Number of pages8
JournalExperimental and Clinical Immunogenetics
Volume14
Issue number2
Publication statusPublished - Oct 1997

Keywords

  • HLA-DR epitopes
  • Phage library
  • Single-chain antibody fragments

ASJC Scopus subject areas

  • Immunology
  • Genetics(clinical)
  • Genetics

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    Pistillo, M. P., Hammer, J., Bono, E., Sinigaglia, F., Gho, A., Marchisone, C., & Ferrara, G. B. (1997). Isolation of scFv fragments to polymorphic and monomorphic HLA-DR epitopes from a synthetic phage library. Experimental and Clinical Immunogenetics, 14(2), 123-130.