Isolation of spindle-shaped cell populations from primary cultures of Kaposi's sarcoma of different stage

Roberto Benelli, Adriana Albini, Carlo Parravicini, Sebastiano Carlone, Lazzaro Repetto, Giuseppe Tambussi, Adriano Lazzarin

Research output: Contribution to journalArticlepeer-review

Abstract

Cell lines derived both from sporadic and epidemic KS biopsies show similar characteristics: a mixture of mesenchymal and vascular markers as well as production of factors which recruit endothelial cells in vitro and induce neoangiogenesis in vivo. Most established KS spindle cell strains are derived from patch or plaque stage KS lesions, which are easily collected during routine biopsies. Here we have characterized KS-derived spindle cell lines obtained from the four different stages typical of KS progression: angiomatous macula, patch, plaque and nodular KS to show if the similar features of our KS cell lines are linked to a particular stage of progression or to an in vitro selection/differentiation during KS cell culture. These four KS cell lines have shown the same pattern of characterization as the previous established KS cell lines, apart from an early selection of the spindle cell population we have also observed an easy inducible phenotypic differentiation through a myofibroblastic spindle cell type simply plating cells on gelatin-coated flasks. These data confirm the hypothesis of spindle cell selection in culture and the possible differentiation of these mesenchymal cells.

Original languageEnglish
Pages (from-to)125-132
Number of pages8
JournalCancer Letters
Volume100
Issue number1-2
DOIs
Publication statusPublished - Feb 27 1996

Keywords

  • Angiogenesis
  • Cell culture
  • Kaposi's sarcoma
  • Kaposi's sarcoma stages

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology
  • Oncology

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