Isolation of wild-type hepatitis A virus from the environment

M. Divizia, P. de Filippis, A. Di Napoli, A. Venuti, R. Perez-Bercoff, A. Pana

Research output: Contribution to journalArticlepeer-review

Abstract

Three isolates of human hepatitis A virus (HAV) were cultivated directly from polluted Tiber water on Frp/3 cells. From 50 to 125 l. of water were prefiltered through 10 and 1 μm polygard filters and concentrated using a molecular ultrafiltration system. The prefilters were washed with 500 ml of beef-extract pH 9.0-9.5 to remove any adsorbed virus. The washing buffer was acid-pH precipitated or concentrated by ultrafiltration. Each sample was neutralized with a pool of antibodies against all the enteroviruses excluding hepatitis A virus. Three samples obtained from prefilters were scored as positive. Hepatitis A virus was identified by commercial ELISA test, indirect immunofluorescence, neutralization test, performed with polyclonal or monoclonal antibody, and by hybridization test with cDNA probes.

Original languageEnglish
Pages (from-to)1155-1160
Number of pages6
JournalWater Research
Volume23
Issue number9
DOIs
Publication statusPublished - 1989

Keywords

  • cDNA probes
  • hepatitis A
  • isolation
  • molecular filtration
  • monoclonal antibody
  • neutralization

ASJC Scopus subject areas

  • Earth-Surface Processes

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