Isomyosin changes after functional electrostimulation of denervated sheep muscle

U. Carraro, C. Catani, L. Saggin, M. Zrunek, M. Szabolcs, H. Gruber, W. Streinzer, W. Mayr, H. Thoma

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Isomyosin analyses by biochemical, immunochemical, and histochemical investigations have been carried out in five sheep following unilateral recurrent laryngeal nerve paralysis and direct functional electrostimulation of the denervated cricoarytenoid posterior muscle. Myosin light chains were identified by two-dimensional gel electrophoresis. Myosin heavy chains were analyzed by one-dimensional SDS-polyacrylamide gel electrophoresis. Slow myosin heavy chain was identified by orthogonal pepitde mapping and immunochemistry. The stimulation effect at cellular level was determined using adenosine triphosphatase (ATPase) histochemistry. A dramatic increase of the type 1 fiber area (slow, fatique-resistant fibers) could be seen after many weeks of an increasing regime of low-frequency direct electrical stimulation. Biochemically, the amount of slow myosin was always higher than in normal muscles. Some muscles were transformed almost completely to the slow type. At the time they were studied and with the methods employed, the expression of embryonic isomyosin was not observed. In conclusion, after numerous weeks of maintained functional activity, elicited by direct electrostimulation, the denervated muscle regionally showed areas of hypertrophy or at least lack of atrophy of slow myofibers without major signs of muscle damage.

Original languageEnglish
Pages (from-to)1016-1028
Number of pages13
JournalMuscle and Nerve
Volume11
Issue number10
Publication statusPublished - 1988

Fingerprint

Sheep
Muscles
Myosin Heavy Chains
Laryngeal Muscles
Immunochemistry
Recurrent Laryngeal Nerve
Vocal Cord Paralysis
Myosin Light Chains
Electrophoresis, Gel, Two-Dimensional
Myosins
Hypertrophy
Electric Stimulation
Atrophy
Adenosine Triphosphatases
Polyacrylamide Gel Electrophoresis

ASJC Scopus subject areas

  • Clinical Neurology
  • Neuroscience(all)

Cite this

Carraro, U., Catani, C., Saggin, L., Zrunek, M., Szabolcs, M., Gruber, H., ... Thoma, H. (1988). Isomyosin changes after functional electrostimulation of denervated sheep muscle. Muscle and Nerve, 11(10), 1016-1028.

Isomyosin changes after functional electrostimulation of denervated sheep muscle. / Carraro, U.; Catani, C.; Saggin, L.; Zrunek, M.; Szabolcs, M.; Gruber, H.; Streinzer, W.; Mayr, W.; Thoma, H.

In: Muscle and Nerve, Vol. 11, No. 10, 1988, p. 1016-1028.

Research output: Contribution to journalArticle

Carraro, U, Catani, C, Saggin, L, Zrunek, M, Szabolcs, M, Gruber, H, Streinzer, W, Mayr, W & Thoma, H 1988, 'Isomyosin changes after functional electrostimulation of denervated sheep muscle', Muscle and Nerve, vol. 11, no. 10, pp. 1016-1028.
Carraro U, Catani C, Saggin L, Zrunek M, Szabolcs M, Gruber H et al. Isomyosin changes after functional electrostimulation of denervated sheep muscle. Muscle and Nerve. 1988;11(10):1016-1028.
Carraro, U. ; Catani, C. ; Saggin, L. ; Zrunek, M. ; Szabolcs, M. ; Gruber, H. ; Streinzer, W. ; Mayr, W. ; Thoma, H. / Isomyosin changes after functional electrostimulation of denervated sheep muscle. In: Muscle and Nerve. 1988 ; Vol. 11, No. 10. pp. 1016-1028.
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