Isoniazid in patient plasma may cause a false-positive result on the complement-dependent cytotoxicity test

Francesca Poli, Annalisa Innocente, Nicoletta Cagni, Caterina Brambilla, Loretta Crespiatico, Maria Bernadette Colombo, Mario Scalamogna

Research output: Contribution to journalArticle

Abstract

Correct definition of clinically relevant anti-HLA antibodies is important for transplant organ allocation and outcome. We describe a candidate for kidney transplantation who was treated with isoniazid because of active tuberculosis. The patient's serum gave a positive antibody result on screening with the complement-dependent cytotoxicity (CDC) test but a negative result on screening with a bead-based assay (Luminex). The clinical history indicated no immunologic stimuli. Subsequent testing on fresh serum samples confirmed the discrepancy between CDC and Luminex results. An autologous cross-match test gave negative results, and the antibodies were sensitive to dithiothreitol treatment. We postulated that nonspecific binding of drug-antibody complexes to panel lymphocytes in the CDC test may have caused the observed lympholysis. This case, although isolated, emphasizes the importance of the combined use of CDC and solid phase assays. The CDC results alone would have led to the erroneous conclusion that the patient was highly sensitized.

Original languageEnglish
Pages (from-to)758-759
Number of pages2
JournalHuman Immunology
Volume70
Issue number9
DOIs
Publication statusPublished - Sep 2009

Keywords

  • Anti-HLA antibodies
  • Isoniazid
  • Lymphocytotoxicity

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Fingerprint Dive into the research topics of 'Isoniazid in patient plasma may cause a false-positive result on the complement-dependent cytotoxicity test'. Together they form a unique fingerprint.

  • Cite this

    Poli, F., Innocente, A., Cagni, N., Brambilla, C., Crespiatico, L., Colombo, M. B., & Scalamogna, M. (2009). Isoniazid in patient plasma may cause a false-positive result on the complement-dependent cytotoxicity test. Human Immunology, 70(9), 758-759. https://doi.org/10.1016/j.humimm.2009.06.012