Isotope labeling-based quantitative proteomics of developing seeds of castor oil seed (Ricinus communis L.)

Fábio C S Nogueira, Giuseppe Palmisano, Veit Schwämmle, Emanuela L. Soares, Arlete A. Soares, Peter Roepstorff, Gilberto B. Domont, Francisco A P Campos

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

In this study, we used a mass spectrometry-based quantification approach employing isotopic (ICPL) and isobaric (iTRAQ) labeling to investigate the pattern of protein deposition during castor oil seed (Ricinus communis L.) development, including that of proteins involved in fatty acid metabolism, seed-storage proteins (SSPs), toxins, and allergens. Additionally, we have used off-line hydrophilic interaction chromatography (HILIC) as a step of peptide fractionation preceding the reverse-phase nanoLC coupled to a LTQ Orbitrap. We were able to identify a total of 1875 proteins, and from these 1748 could be mapped to extant castor gene models, considerably expanding the number of proteins so far identified from developing castor seeds. Cluster validation and statistical analysis resulted in 975 protein trend patterns and the relative abundance of 618 proteins. The results presented in this work give important insights into certain aspects of the biology of castor oil seed development such as carbon flow, anabolism, and catabolism of fatty acid and the pattern of deposition of SSPs, toxins, and allergens such as ricin and 2S albumins. We also found, for the first time, some genes of SSP that are differentially expressed during seed development.

Original languageEnglish
Pages (from-to)5012-5024
Number of pages13
JournalJournal of Proteome Research
Volume12
Issue number11
DOIs
Publication statusPublished - Nov 1 2013

Fingerprint

Castor Oil
Isotope Labeling
Ricinus
Oilseeds
Isotopes
Proteomics
Labeling
Seed
Seeds
Seed Storage Proteins
Proteins
Allergens
Fatty Acids
Genes
Ricin
Cluster analysis
Fractionation
Chromatography
Hydrophobic and Hydrophilic Interactions
Metabolism

Keywords

  • ICPL
  • iTRAQ
  • quantitative plant proteomics
  • Ricinus communis
  • seed development

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

Cite this

Nogueira, F. C. S., Palmisano, G., Schwämmle, V., Soares, E. L., Soares, A. A., Roepstorff, P., ... Campos, F. A. P. (2013). Isotope labeling-based quantitative proteomics of developing seeds of castor oil seed (Ricinus communis L.). Journal of Proteome Research, 12(11), 5012-5024. https://doi.org/10.1021/pr400685z

Isotope labeling-based quantitative proteomics of developing seeds of castor oil seed (Ricinus communis L.). / Nogueira, Fábio C S; Palmisano, Giuseppe; Schwämmle, Veit; Soares, Emanuela L.; Soares, Arlete A.; Roepstorff, Peter; Domont, Gilberto B.; Campos, Francisco A P.

In: Journal of Proteome Research, Vol. 12, No. 11, 01.11.2013, p. 5012-5024.

Research output: Contribution to journalArticle

Nogueira, FCS, Palmisano, G, Schwämmle, V, Soares, EL, Soares, AA, Roepstorff, P, Domont, GB & Campos, FAP 2013, 'Isotope labeling-based quantitative proteomics of developing seeds of castor oil seed (Ricinus communis L.)', Journal of Proteome Research, vol. 12, no. 11, pp. 5012-5024. https://doi.org/10.1021/pr400685z
Nogueira FCS, Palmisano G, Schwämmle V, Soares EL, Soares AA, Roepstorff P et al. Isotope labeling-based quantitative proteomics of developing seeds of castor oil seed (Ricinus communis L.). Journal of Proteome Research. 2013 Nov 1;12(11):5012-5024. https://doi.org/10.1021/pr400685z
Nogueira, Fábio C S ; Palmisano, Giuseppe ; Schwämmle, Veit ; Soares, Emanuela L. ; Soares, Arlete A. ; Roepstorff, Peter ; Domont, Gilberto B. ; Campos, Francisco A P. / Isotope labeling-based quantitative proteomics of developing seeds of castor oil seed (Ricinus communis L.). In: Journal of Proteome Research. 2013 ; Vol. 12, No. 11. pp. 5012-5024.
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