Isotype switching and titer variation of anti-Ro/SSA antibodies over time in 100 patients with undifferentiated connective tissue disease (UCTD)

Angela Ceribelli, Ilaria Cavazzana, Franco Franceschini, Marzia Quinzanini, Fabio Lodi Rizzini, Roberto Cattaneo

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Objective. To correlate the clinical course of the disease with the titer, the isotype profile and the switch of the anti-Ro/SSA antibodies in a cohort of patients affected by UCTD. Methods. One hundred selected patients with anti-Ro/SSA antibodies detected by counterimmunoelectrophoresis (CIE), and affected by UCTD with a mean follow-up of 7.6 years (SD 4.8 yrs.), were studied. The titer of IgA, IgG and IgM anti-Ro/SSA antibodies was determined in two different sera, obtained at the time of diagnosis and at the last visit, by ELISA with Ro/SSA recombinant proteins as substrate. Results. Thirty-five patients evolved from UCTD to a different connective tissue disease, while 65 showed a stable disease. Anti-Ro/SSA antibodies were detected in 91% and 97% of the patients, at baseline and during follow-up, respectively. IgG dominates the anti-Ro response. The titer of IgA, IgM and IgG anti-Ro/SSA did not differ significantly between the two groups of patients with UCTD. An increasing trend of IgG and IgA anti-Ro/SSA titer could be detected in patients evolving in primary Sjögren's Syndrome (pSS), but only the increase of IgG anti-Ro/SSA was significant (p = 0.0235). Conclusion. IgG dominates the anti-Ro/SSA response in patients with UCTD. No substantial change of the antibody isotype against Ro/SSA peptides could be observed during follow-up. The titer of IgG anti-Ro/ SSA significantly raised in the group of patients evolving in pSS.

Original languageEnglish
Pages (from-to)117-120
Number of pages4
JournalClinical and Experimental Rheumatology
Volume26
Issue number1
Publication statusPublished - Jan 2008

Fingerprint

Immunoglobulin Class Switching
Connective Tissue Diseases
Counterimmunoelectrophoresis
SS-A antibodies
anti-IgG
Recombinant Proteins
Enzyme-Linked Immunosorbent Assay
Peptides
Antibodies

Keywords

  • Anti-Ro/SSA antibodies
  • Antibody titer
  • Isotype switching
  • Sjögren's syndrome
  • Systemic lupus erythematosus
  • Undifferentiated connective tissue disease

ASJC Scopus subject areas

  • Rheumatology
  • Immunology

Cite this

Isotype switching and titer variation of anti-Ro/SSA antibodies over time in 100 patients with undifferentiated connective tissue disease (UCTD). / Ceribelli, Angela; Cavazzana, Ilaria; Franceschini, Franco; Quinzanini, Marzia; Rizzini, Fabio Lodi; Cattaneo, Roberto.

In: Clinical and Experimental Rheumatology, Vol. 26, No. 1, 01.2008, p. 117-120.

Research output: Contribution to journalArticle

Ceribelli, Angela ; Cavazzana, Ilaria ; Franceschini, Franco ; Quinzanini, Marzia ; Rizzini, Fabio Lodi ; Cattaneo, Roberto. / Isotype switching and titer variation of anti-Ro/SSA antibodies over time in 100 patients with undifferentiated connective tissue disease (UCTD). In: Clinical and Experimental Rheumatology. 2008 ; Vol. 26, No. 1. pp. 117-120.
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AU - Rizzini, Fabio Lodi

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N2 - Objective. To correlate the clinical course of the disease with the titer, the isotype profile and the switch of the anti-Ro/SSA antibodies in a cohort of patients affected by UCTD. Methods. One hundred selected patients with anti-Ro/SSA antibodies detected by counterimmunoelectrophoresis (CIE), and affected by UCTD with a mean follow-up of 7.6 years (SD 4.8 yrs.), were studied. The titer of IgA, IgG and IgM anti-Ro/SSA antibodies was determined in two different sera, obtained at the time of diagnosis and at the last visit, by ELISA with Ro/SSA recombinant proteins as substrate. Results. Thirty-five patients evolved from UCTD to a different connective tissue disease, while 65 showed a stable disease. Anti-Ro/SSA antibodies were detected in 91% and 97% of the patients, at baseline and during follow-up, respectively. IgG dominates the anti-Ro response. The titer of IgA, IgM and IgG anti-Ro/SSA did not differ significantly between the two groups of patients with UCTD. An increasing trend of IgG and IgA anti-Ro/SSA titer could be detected in patients evolving in primary Sjögren's Syndrome (pSS), but only the increase of IgG anti-Ro/SSA was significant (p = 0.0235). Conclusion. IgG dominates the anti-Ro/SSA response in patients with UCTD. No substantial change of the antibody isotype against Ro/SSA peptides could be observed during follow-up. The titer of IgG anti-Ro/ SSA significantly raised in the group of patients evolving in pSS.

AB - Objective. To correlate the clinical course of the disease with the titer, the isotype profile and the switch of the anti-Ro/SSA antibodies in a cohort of patients affected by UCTD. Methods. One hundred selected patients with anti-Ro/SSA antibodies detected by counterimmunoelectrophoresis (CIE), and affected by UCTD with a mean follow-up of 7.6 years (SD 4.8 yrs.), were studied. The titer of IgA, IgG and IgM anti-Ro/SSA antibodies was determined in two different sera, obtained at the time of diagnosis and at the last visit, by ELISA with Ro/SSA recombinant proteins as substrate. Results. Thirty-five patients evolved from UCTD to a different connective tissue disease, while 65 showed a stable disease. Anti-Ro/SSA antibodies were detected in 91% and 97% of the patients, at baseline and during follow-up, respectively. IgG dominates the anti-Ro response. The titer of IgA, IgM and IgG anti-Ro/SSA did not differ significantly between the two groups of patients with UCTD. An increasing trend of IgG and IgA anti-Ro/SSA titer could be detected in patients evolving in primary Sjögren's Syndrome (pSS), but only the increase of IgG anti-Ro/SSA was significant (p = 0.0235). Conclusion. IgG dominates the anti-Ro/SSA response in patients with UCTD. No substantial change of the antibody isotype against Ro/SSA peptides could be observed during follow-up. The titer of IgG anti-Ro/ SSA significantly raised in the group of patients evolving in pSS.

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