IV. Potential impact of Pneumocystis genetic diversity on the molecular detection of the parasite in human host

Enrica Tamburrini, Paola Mencarini, Elena Visconti, Maria Zolfo, Salvatore Marinaci, Daniela Zinzi, Paola Margutti, Elena Ortona, Alessandra Siracusano

Research output: Contribution to journalArticle

Abstract

Our aim was to evaluate if genetic diversity of Pneumocystis carinii could influence the detection by molecular techniques in bronchoalveolar lavage (BAL) fluids and in non-invasive specimens (induced sputum, oropharyngeal washing and serum/blood). P. carinii is morphologically similar in different hosts although several strains have been identified by biomolecular techniques. Variations of mt-LSU and ITSs sequences could determine a lack of hybridization of some clinical samples and could have diagnostic consequences with loss in sensitivity and specificity of available molecular tests, but at the moment no data support a significant impact of genetic diversity in these sequences on molecular detection of P. carinii for clinical purposes. Copyright (C) 1998 Federation of European Microbiological Societies.

Original languageEnglish
Pages (from-to)37-49
Number of pages13
JournalFEMS Immunology and Medical Microbiology
Volume22
Issue number1-2
DOIs
Publication statusPublished - Sep 1998

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Keywords

  • Genetic polymorphisms
  • ITS
  • mt-LSU
  • PCP
  • Pneumocystis carinii
  • Respiratory samples

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Infectious Diseases

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