JC virus load in cerebrospinal fluid and transcriptional control region rearrangements may predict the clinical course of progressive multifocal leukoencephalopathy

Serena Delbue, Francesca Elia, Camilla Carloni, Eleonora Tavazzi, Enrico Marchioni, Silvia Carluccio, Lucia Signorini, Stefano Novati, Renato Maserati, Pasquale Ferrante

Research output: Contribution to journalArticle

Abstract

Progressive multifocal leukoencephalopathy (PML) is a severe disease of the central nervous system (CNS), caused by infection with the Polyomavirus JC virus (JCV). Because there are no known treatments or prognostic factors, we performed a long-term study focusing mainly on cerebrospinal fluid (CSF) samples from PML patients to describe the virological features akin to the different forms of the disease. Twenty-eight PML patients were enrolled: 10 HIV-1+ patients with classical PML (CPML), 9 HIV-1+ patients with slowly progressing or stable neurological symptoms (benign PML), 3 HIV-1+ asymptomatic patients, and 6 HIV-1-negative patients. CSF, urine, and blood samples were collected at the enrollment (baseline) and every 6 months afterwards when possible. The JCV DNA and HIV-1 RNA loads were determined, and the JCV strains were characterized. At baseline, the mean CSF JCV load was log6.0±1.2copies/ml for CPML patients, log4.0±1.0 copies/ml for benign PML patients, log4.2±0.5 copies/ml for asymptomatic PML patients, and log5.8±1.3copies/ml for HIV-1-negative PML patients (CPML vs. benign: P

Original languageEnglish
Pages (from-to)3511-3517
Number of pages7
JournalJournal of Cellular Physiology
Volume227
Issue number10
DOIs
Publication statusPublished - Oct 2012

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ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

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