JCV/BKV and SV40 viral load in lymphoid tissues of young immunocompetent children from an area of north-east Italy

Manola Comar, Nunzia Zanotta, Massimo Bovenzi, Cesare Campello

Research output: Contribution to journalArticle

Abstract

Polyomavirus infection occurring during childhood is followed by a lifelong latency in immunocompetent subjects. The major site of polyomavirus persistence are the uroepithelial cells which leads to oral transmission. It has recently been hypothesized that tonsils could be a possible reservoir. The role of tonsil, adenoid, and peripheral blood mononuclear cells (PBMCs) as possible sites of JCV, BKV, and SV40 latency in young healthy children was assessed. Two hundred fifteen fresh specimens, including 57 tonsil, 80 adenoid, and 78 PBMC samples from 80 immunocompetent children (mean age 4.8 years) were analyzed to determine the viral load by quantitative real-time PCR. The human herpes virus 6 (HHV-6) was tested as a lymphotropic reference virus. Polyomavirus was detected in 5/ 80 (6.2%) children while HHV-6 infection affected 27/80 children (33.7%) (P2/5.5 × 10e4 to 6.8 × 10e3/8.5 × 10e4 viral copies/number of cells. In conclusion, tonsils and adenoids of children could effectively harbor BKV and SV40, although only very few cells proved to be infected. Nevertheless, the low prevalence of polyomavirus, in comparison with the lymphotropic HHV-6, suggests that these tissues are unlikely to be the preferred site of polyomavirus latency, at least in younger children.

Original languageEnglish
Pages (from-to)1236-1240
Number of pages5
JournalJournal of Medical Virology
Volume82
Issue number7
DOIs
Publication statusPublished - 2010

Keywords

  • Adenoid
  • Children
  • HHV-6
  • Latency
  • Polyomavirus
  • Tonsil

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases
  • Medicine(all)

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