K Index in cerebrospinal fluid: a valid tool in multiple sclerosis diagnosis

Massimo Pieri, Fabio Duranti, Diego Centonze, Fabio Buttari, Sergio Bernardini, Mariarita Dessì

Research output: Contribution to journalArticlepeer-review

Abstract

Background.: Detection of oligoclonal IgG bands in cerebrospinal fluid by isoelectrocfocusing and immunodetection is the current gold standard to detect an inflammatory process in the central nervous system. It has been proposed that the presence of free light chains (FLCs) in CSF was associated with recent demyelination activity in MS and might be used as a prognosis marker. Our study’s objective is assessing the diagnostic accuracy of a new highly sensitive latex-enhanced nephelometric assay for k free light chain (kFLC) determination in CSF/serum as an alternative to traditional tests and its clinical application.

Methods.: kFLCs were measured in CSF/serum pairs from 80 patients by the use of a new highly sensitive latex-enhanced nephelometric automated immunoassay for detection of immunoglobulin FLC. The eighty patients were split into three groups according to the neurological diagnosis. In this study we confirm even more the use of the k Index as a diagnostic aid in multiple sclerosis.

Results.: kFLC Index seems to be more accurate parameter respect the determination of oligoclonal immunoglobulin bands (OCBs). We recalculate the K Index sensitivity and specificity respect the precedent published result. Two patients previously diagnosed with leukoencephalopathy have gone to group 3 as confirmed the diagnosis of MS.

Conclusions.: These new data reinforce even more the use of the k Index to diagnose MS in comparison to classical methods and to the reference method, the OCBs.

Original languageEnglish
Pages (from-to)167-171
Number of pages5
JournalRivista Italiana della Medicina di Laboratorio
Volume10
Issue number3
DOIs
Publication statusPublished - Oct 31 2014

Keywords

  • CSF
  • kFLC Index
  • kFLCs
  • Multiple sclerosis
  • Nephelometry
  • OCBs

ASJC Scopus subject areas

  • Biochemistry, medical
  • Medical Laboratory Technology

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