Kaposi's sarcoma-associated herpesvirus DNA sequences in AIDS-related and AIDS-unrelated lymphomatous effusions

Antonino Carbone, Annunziata Gloghini, Emanuela Vaccher, Vittorina Zagonel, Cristina Pastore, Paolo Dalla Palma, Fabio Branz, Giuseppe Saglio, Rachele Volpe, Umberto Tirelli, Gianluca Gaidano

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Abstract

Primary effusions presenting as the sole lymphoma localization are also known as body-cavity-based-lymphoma (BCBL), and have been shown to carry Kaposi's sarcoma herpesvirus (KSHV) DNA sequences. The aim of this study was a comparative analysis of the clinical, pathologic and molecular features of BCBL and lymphomatous effusions secondary to tissue-based lymphomas occurring both in the general population and in HIV-infected individuals. All the lymphomatous effusion samples (seven AIDS-related and nine AIDS-unrelated) were subjected to an identical multiparameter investigation, including collection of clinical data, analysis of morphology and immunophenotype, as well as the study of viral sequences and genetic lesions. In six cases defined as BCBL (four AIDS-related and two AIDS-unrelated), the patients exhibited exclusive or predominant involvement of the body cavities. BCBL tended to display indeterminate phenotypes (4/6), whereas all AIDS-related and AIDS-unrelated lymphomatous effusions secondary to tissue-based lymphomas consistently expressed B-cell phenotype. Detection of KSHV DNA sequences was restricted to cases of BCBL (3/4 AIDS-related and 1/2 AIDS-unrelated), whereas EBV association (3/4) and expression of EBV-encoded antigens (LMP-1, 2/3; EBNA-2, 1/3) were confined to the AIDS-related BCBL. Overall, our results confirm that both AIDS-related and AIDS-unrelated BCBL preferentially associate with peculiar clinical, immunophenotypic and molecular features among lymphomatous effusions and therefore should be singled out as a specific clinico-pathologic entity.

Original languageEnglish
Pages (from-to)533-543
Number of pages11
JournalBritish Journal of Haematology
Volume94
Issue number3
Publication statusPublished - 1996

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Keywords

  • AIDS-related lymphomatous effusions
  • AIDS-unrelated lymphomtous effusions
  • Body-cavity-based lymphoma
  • HIV-8
  • Kaposi's sarcoma herpesvirus (KSHV)

ASJC Scopus subject areas

  • Hematology

Cite this

Carbone, A., Gloghini, A., Vaccher, E., Zagonel, V., Pastore, C., Palma, P. D., Branz, F., Saglio, G., Volpe, R., Tirelli, U., & Gaidano, G. (1996). Kaposi's sarcoma-associated herpesvirus DNA sequences in AIDS-related and AIDS-unrelated lymphomatous effusions. British Journal of Haematology, 94(3), 533-543.