Kaposi's sarcoma cells express the macrophage-associated antigen mannose receptor and develop in peripheral blood cultures of Kaposi's sarcoma patients

Stefania Uccini, Maria C. Sirianni, Laura Vincenzi, Simone Topino, Antonella Stoppacciaro, Ilaria Lesnoni La Parola, Maria Capuano, Cinzia Masini, Decio Cerimele, Marina Cella, Antonio Lanzavecchia, Paola Allavena, Alberto Mantovani, Carlo D. Baroni, Luigi P. Ruco

Research output: Contribution to journalArticle

Abstract

The mannose receptor (MR) is a surface 175-kd C-type lectin expressed by macrophages and dendritic cells. MR is involved in a removal of effete cells, phagocytosis of mannose-coated particles, pinocytosis, and antigen presentation. Expression of MR was investigated in 17 biopsies of Kaposi's sarcoma (3 AIDS KS, 13 classical KS, and 1 transplant-associated KS) using three anti-MR monoclonal antibodies (3.29, D547, and PAM1). Immunostaining for MR was detected in 94 ± 7% KS cells with spindle morphology. In normal tissues, MR was expressed by sinus-lining cells of spleen and lymph nodes, but it was not detected in endothelial cells lining normal hematic and lymphatic vessels, hemangioma, hemangioendothelioma, and lymphangioma. Expression of MR in KS cells prompted us to investigate the possibility that they derive from a circulating precursor cell. Peripheral blood mononuclear cells from 16 patients with KS (10 classical, 1 transplanted, and 5 AIDS) were cultured in PHA-conditioned medium for 10 to 14 days. Confluent monolayers of adherent spindle cells were detected in 8 of 11 classical KS, in 5 of 5 AIDS KS patients, and in 0 of 34 control patients. Peripheral- blood-derived KS-like cells were characterized by co-expression of macrophage and endothelial antigens being positive for CD45 (60%), CD68 (98%), MR (70%), CD14 (25%), VE-cadherin (70%), and von Willebrand factor (10%). When the immunophenotype of peripheral-blood-derived adherent cells was compared with that of KS spindle cells of tissue biopsies, it was found that both cell types are VE-cadherin+/MR+/CD68+, that peripheral-blood-derived spindle cells are CD34- and are less frequently stained for CD31 and von Willebrand factor, and that lesional KS cells do not express the leukocyte markers CD45 and CD18. Our findings are consistent with the possibility that KS lesions derive from tissue accumulation and local proliferation of a special subset of macrophages with endothelial features the normal counterpart of which are the sinus-lining cells of spleen and lymph nodes.

Original languageEnglish
Pages (from-to)929-938
Number of pages10
JournalAmerican Journal of Pathology
Volume150
Issue number3
Publication statusPublished - 1997

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Antigen Receptors
Kaposi's Sarcoma
Macrophages
Acquired Immunodeficiency Syndrome
von Willebrand Factor
Blood Culture
mannose receptor
Spleen
Lymph Nodes
Pinocytosis
Hemangioendothelioma
C-Type Lectins
Biopsy
Lymphangioma
Cytophagocytosis
Lymphatic Vessels
Antigen Presentation
Hemangioma
Mannose
Conditioned Culture Medium

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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Kaposi's sarcoma cells express the macrophage-associated antigen mannose receptor and develop in peripheral blood cultures of Kaposi's sarcoma patients. / Uccini, Stefania; Sirianni, Maria C.; Vincenzi, Laura; Topino, Simone; Stoppacciaro, Antonella; La Parola, Ilaria Lesnoni; Capuano, Maria; Masini, Cinzia; Cerimele, Decio; Cella, Marina; Lanzavecchia, Antonio; Allavena, Paola; Mantovani, Alberto; Baroni, Carlo D.; Ruco, Luigi P.

In: American Journal of Pathology, Vol. 150, No. 3, 1997, p. 929-938.

Research output: Contribution to journalArticle

Uccini, S, Sirianni, MC, Vincenzi, L, Topino, S, Stoppacciaro, A, La Parola, IL, Capuano, M, Masini, C, Cerimele, D, Cella, M, Lanzavecchia, A, Allavena, P, Mantovani, A, Baroni, CD & Ruco, LP 1997, 'Kaposi's sarcoma cells express the macrophage-associated antigen mannose receptor and develop in peripheral blood cultures of Kaposi's sarcoma patients', American Journal of Pathology, vol. 150, no. 3, pp. 929-938.
Uccini, Stefania ; Sirianni, Maria C. ; Vincenzi, Laura ; Topino, Simone ; Stoppacciaro, Antonella ; La Parola, Ilaria Lesnoni ; Capuano, Maria ; Masini, Cinzia ; Cerimele, Decio ; Cella, Marina ; Lanzavecchia, Antonio ; Allavena, Paola ; Mantovani, Alberto ; Baroni, Carlo D. ; Ruco, Luigi P. / Kaposi's sarcoma cells express the macrophage-associated antigen mannose receptor and develop in peripheral blood cultures of Kaposi's sarcoma patients. In: American Journal of Pathology. 1997 ; Vol. 150, No. 3. pp. 929-938.
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abstract = "The mannose receptor (MR) is a surface 175-kd C-type lectin expressed by macrophages and dendritic cells. MR is involved in a removal of effete cells, phagocytosis of mannose-coated particles, pinocytosis, and antigen presentation. Expression of MR was investigated in 17 biopsies of Kaposi's sarcoma (3 AIDS KS, 13 classical KS, and 1 transplant-associated KS) using three anti-MR monoclonal antibodies (3.29, D547, and PAM1). Immunostaining for MR was detected in 94 ± 7{\%} KS cells with spindle morphology. In normal tissues, MR was expressed by sinus-lining cells of spleen and lymph nodes, but it was not detected in endothelial cells lining normal hematic and lymphatic vessels, hemangioma, hemangioendothelioma, and lymphangioma. Expression of MR in KS cells prompted us to investigate the possibility that they derive from a circulating precursor cell. Peripheral blood mononuclear cells from 16 patients with KS (10 classical, 1 transplanted, and 5 AIDS) were cultured in PHA-conditioned medium for 10 to 14 days. Confluent monolayers of adherent spindle cells were detected in 8 of 11 classical KS, in 5 of 5 AIDS KS patients, and in 0 of 34 control patients. Peripheral- blood-derived KS-like cells were characterized by co-expression of macrophage and endothelial antigens being positive for CD45 (60{\%}), CD68 (98{\%}), MR (70{\%}), CD14 (25{\%}), VE-cadherin (70{\%}), and von Willebrand factor (10{\%}). When the immunophenotype of peripheral-blood-derived adherent cells was compared with that of KS spindle cells of tissue biopsies, it was found that both cell types are VE-cadherin+/MR+/CD68+, that peripheral-blood-derived spindle cells are CD34- and are less frequently stained for CD31 and von Willebrand factor, and that lesional KS cells do not express the leukocyte markers CD45 and CD18. Our findings are consistent with the possibility that KS lesions derive from tissue accumulation and local proliferation of a special subset of macrophages with endothelial features the normal counterpart of which are the sinus-lining cells of spleen and lymph nodes.",
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T1 - Kaposi's sarcoma cells express the macrophage-associated antigen mannose receptor and develop in peripheral blood cultures of Kaposi's sarcoma patients

AU - Uccini, Stefania

AU - Sirianni, Maria C.

AU - Vincenzi, Laura

AU - Topino, Simone

AU - Stoppacciaro, Antonella

AU - La Parola, Ilaria Lesnoni

AU - Capuano, Maria

AU - Masini, Cinzia

AU - Cerimele, Decio

AU - Cella, Marina

AU - Lanzavecchia, Antonio

AU - Allavena, Paola

AU - Mantovani, Alberto

AU - Baroni, Carlo D.

AU - Ruco, Luigi P.

PY - 1997

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N2 - The mannose receptor (MR) is a surface 175-kd C-type lectin expressed by macrophages and dendritic cells. MR is involved in a removal of effete cells, phagocytosis of mannose-coated particles, pinocytosis, and antigen presentation. Expression of MR was investigated in 17 biopsies of Kaposi's sarcoma (3 AIDS KS, 13 classical KS, and 1 transplant-associated KS) using three anti-MR monoclonal antibodies (3.29, D547, and PAM1). Immunostaining for MR was detected in 94 ± 7% KS cells with spindle morphology. In normal tissues, MR was expressed by sinus-lining cells of spleen and lymph nodes, but it was not detected in endothelial cells lining normal hematic and lymphatic vessels, hemangioma, hemangioendothelioma, and lymphangioma. Expression of MR in KS cells prompted us to investigate the possibility that they derive from a circulating precursor cell. Peripheral blood mononuclear cells from 16 patients with KS (10 classical, 1 transplanted, and 5 AIDS) were cultured in PHA-conditioned medium for 10 to 14 days. Confluent monolayers of adherent spindle cells were detected in 8 of 11 classical KS, in 5 of 5 AIDS KS patients, and in 0 of 34 control patients. Peripheral- blood-derived KS-like cells were characterized by co-expression of macrophage and endothelial antigens being positive for CD45 (60%), CD68 (98%), MR (70%), CD14 (25%), VE-cadherin (70%), and von Willebrand factor (10%). When the immunophenotype of peripheral-blood-derived adherent cells was compared with that of KS spindle cells of tissue biopsies, it was found that both cell types are VE-cadherin+/MR+/CD68+, that peripheral-blood-derived spindle cells are CD34- and are less frequently stained for CD31 and von Willebrand factor, and that lesional KS cells do not express the leukocyte markers CD45 and CD18. Our findings are consistent with the possibility that KS lesions derive from tissue accumulation and local proliferation of a special subset of macrophages with endothelial features the normal counterpart of which are the sinus-lining cells of spleen and lymph nodes.

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