Karyotypic analysis of gastric carcinoma cell lines carrying an amplified c-met oncogene

Giovanna Rege-Cambrin, Patrizia Scaravaglio, Franca Carozzi, Silvia Giordano, Carola Ponzetto, Paolo M. Comoglio, Giuseppe Saglio

Research output: Contribution to journalArticlepeer-review

Abstract

MKN 45 is a poorly differentiated gastric carcinoma cell line from which the subclone GTL 16 was obtained. Both lines carry an amplification unit derived from chromosome 7 sequences and containing an activated c-met oncogene. Karyotypic analysis showed that GTL 16 derived from a subclone of MKN 45 after endoreduplication. Several clonal abnormalities are evident in both lines; some are frequently observed in gastrointestinal tumors (loss of 17p and monosomy 18). Other consistent anomalies include 6q-, t(8;10) and t(5;8), and inv(16). A marker chromosome (M1), which was previously shown to contain the c-met amplification unit, is constantly duplicated in all GTL 16 metaphases; in contrast, most unidentified markers are retained in only a single copy in GTL 16 cells. These data are in agreement with the hypothesis that the c-met oncogene activation in these gastric cancer cell lines might be related to a gene dosage effect.

Original languageEnglish
Pages (from-to)170-173
Number of pages4
JournalCancer Genetics and Cytogenetics
Volume64
Issue number2
DOIs
Publication statusPublished - 1992

ASJC Scopus subject areas

  • Cancer Research
  • Genetics
  • Molecular Biology

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