Keratinocytes from atopic dermatitis patients produce increased amounts of GM-CSF

S. Pastore, E. Fanales-Belasio, C. Albanesi, D. Mei, G. Girolomoni

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Atopic diseases are characterized by important dysregulations in the production of cytokines involved in the control of immune responses. In order to examine the contribution of epidermis to the pathogenesis of atopic dermatitis (AD), keratinocyte cultures were established from uninvolved skin of patients with AD and from healthy subjects, and compared in terms of GM-CSF production. Second- or third-passage cultured atopic keratinocytes exhibited a markedly increased GM-CSF release compared to keratinocytes from healthy controls, both spontaneously and after treatment with PMA. In parallel, RT-PCR analysis showed a higher constitutive as well as PMA-induced GM-CSF gene expression in atopic keratinocytes. Moreover, keratinocyte supernatants were able to reproduce the y of hrGM-CSF on immunophenotypical and functional maturation of peripheral blood monocytes to dendritic cells. An exaggerated production of GM-CSF by atopic keratinocytes may contribute relevantly to the chronicity of AD lesions, in particular to the increased recruitment, sustained activation and enhanced antigen-presenting-cell functions of dendritic cells in AD skin.

Original languageEnglish
Pages (from-to)93-96
Number of pages4
JournalInternational Journal of Immunopathology and Pharmacology
Volume10
Issue number1
Publication statusPublished - 1997

Fingerprint

Atopic Dermatitis
Granulocyte-Macrophage Colony-Stimulating Factor
Keratinocytes
Dendritic Cells
Skin
Antigen-Presenting Cells
Epidermis
Monocytes
Healthy Volunteers
Cytokines
Gene Expression
Polymerase Chain Reaction

Keywords

  • antigen presenting cells
  • atopy
  • epithelial cells
  • GM-CSF
  • immune responses

ASJC Scopus subject areas

  • Pharmacology

Cite this

Keratinocytes from atopic dermatitis patients produce increased amounts of GM-CSF. / Pastore, S.; Fanales-Belasio, E.; Albanesi, C.; Mei, D.; Girolomoni, G.

In: International Journal of Immunopathology and Pharmacology, Vol. 10, No. 1, 1997, p. 93-96.

Research output: Contribution to journalArticle

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