Key components of the fission machinery are interchangeable

Jia Shu Yang, Leiliang Zhang, Stella Y. Lee, Helge Gad, Alberto Luini, Victor W. Hsu

Research output: Contribution to journalArticlepeer-review


Brefeldin-A ADP-ribosylated substrate (BARS) and dynamin function in membrane fission in distinct intracellular transport pathways, but whether their functions are mechanistically similar is unclear. Here, we show that ARFGAP1, a GTPase-activating protein (GAP) for ADP-ribosylation factor 1 (ARF1), couples to either BARS or endophilin B for vesicle formation by the coat protein I (COPI) complex - a finding that reveals an unanticipated mechanistic flexibility in mammalian COPI transport. Because dynamin is coupled to endophilin A in vesicle formation by the clathrin-coat complex, our finding also predicts that dynamin and ARF GAPs are likely to be functional counterparts in membrane fission among different transport pathways that connect intracellular membrane compartments.

Original languageEnglish
Pages (from-to)1376-1382
Number of pages7
JournalNature Cell Biology
Issue number12
Publication statusPublished - Dec 2006

ASJC Scopus subject areas

  • Cell Biology

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