Ki-67 antigert retrieval in formalin- or ethanol-fixed, paraffin-embedded tissues: An enhancement method for immunohistochemical staining with autoclave treatment

Yoshiro Tsuji, Katsuyuki Kusuzaki, Yasusuke Hirasawa, Massimo Serra, Nicola Baldini

Research output: Contribution to journalArticle


We developed a new immunohistochemical method to visualize Ki-67 antigen in formalin-fixed, paraffin-embedded sections of bone tumors. The method uses autoclave pretreatment of sections immersed in citrate buffer before incubation with the MIB 1 antibody, which reacts with the Ki-67 gene product. MIB 1 antibody showed strong and discrete staining of cell nuclei in proliferating tumor cells. This method also retrieved antigen in paraffin-embedded sections of alcohol-fixed tissue. Therefore, tissues previously considered unsuitable for immunohistochemical analysis can be studied after autoclave treatment.

Original languageEnglish
Pages (from-to)251-255
Number of pages5
JournalActa Histochemica et Cytochemica
Issue number3
Publication statusPublished - 1997



  • Autoclave treatment
  • Immunohistochemistry
  • MIB1 antibody

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Endocrinology
  • Anatomy
  • Neuroscience(all)

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